Microbial fingerprinting detects intestinal microbiota dysbiosis in Zebrafish models with chemically-induced enterocolitis

BMC Microbiology

Qingshun Zhao²  Jieshou Li¹  Qiurong Li¹  Chun Tang¹  Chenyang Wang¹  Fan Wang¹  Lin Wang¹  Qi He¹ 

[1] Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, No.305 East Zhongshan Road, Nanjing 210002, China;Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China

关键词: Microbiota;    Trinitrobenzenesulfonic acid;    Denaturing gradient gel electrophoresis;    Zebrafish;   

Others  :  1142409
DOI  :  10.1186/1471-2180-13-289

 received in 2013-05-06, accepted in 2013-10-16,  发布年份 2013

【 摘 要 】

Background

Inflammatory bowel disease (IBD) involves a breakdown in interactions between the host immune response and the resident commensal microbiota. Recent studies have suggested gut physiology and pathology relevant to human IBD can be rapidly modeled in zebrafish larvae. The aim of this study was to investigate the dysbiosis of intestinal microbiota in zebrafish models with IBD-like enterocolitis using culture-independent techniques.

Results

IBD-like enterocolitis was induced by exposing larval zebrafish to trinitrobenzenesulfonic acid (TNBS). Pathology was assessed by histology and immunofluorescence. Changes in intestinal microbiota were evaluated by denaturing gradient gel electrophoresis (DGGE) and the predominant bacterial composition was determined with DNA sequencing and BLAST and confirmed by real-time polymerase chain reaction. Larval zebrafish exposed to TNBS displayed intestinal-fold architecture disruption and inflammation reminiscent of human IBD. In this study, we defined a reduced biodiversity of gut bacterial community in TNBS-induced coliitis. The intestinal microbiota dysbiosis in zebrafish larvae with IBD-like colitis was characterized by an increased proportion of Proteobacteria (especially Burkholderia) and a decreased of Firmicutes(Lactobacillus group), which were significantly correlated with enterocolitis severity(Pearson correlation p < 0.01).

Conclusions

This is the first description of intestinal microbiota dysbiosis in zebrafish IBD-like models, and these changes correlate with TNBS-induced enterocolitis. Prevention or reversal of this dysbiosis may be a viable option for reducing the incidence and severity of human IBD.

【 授权许可】

   
2013 He et al.; licensee BioMed Central Ltd.

【 图 表 】

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