Colostrum supplementation protects against exercise - induced oxidative stress in skeletal muscle in mice

BMC Research Notes

Nagaraja Haleagrahara⁷  Karuthan Chinna³  Poh Bee Koon⁴  Nik Shanita Safii⁴  Mohd Ismail Noor¹  Abu Bakar Abdul Majeed⁵  Rajesh Ramasamy⁸  Kalavathy Ramasamy⁵  Ammu Kutty Radhakrishnan²  Mahenderan Appukutty⁶ 

[1] Faculty of Health Sciences, University Technology Mara, Shah Alam 40450, Kuala Lumpur, Malaysia;Faculty of Medicine and Health, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia;Faculty of Medicine, University Malaya 50603, Kuala Lumpur, Malaysia;Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Bangi 43600, Kuala Lumpur, Malaysia;Faculty of Pharmacy, University Technology Mara, Shah Alam 40450, Kuala Lumpur, Malaysia;Faculty of Sports Science & Recreation, University Technology Mara, Shah Alam 40450, Kuala Lumpur, Malaysia;Nagaraja Haleagrahara, School of Veterinary and Biomedical Sciences, Faculty of Medicine, Health and Molecular Sciences, James Cook University, Townsville 4811, QLD, Australia;Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang 43400, Kuala Lumpur, Malaysia

关键词: Lipid hydroperoxides;    Antioxidants;    Skeletal muscle;    Exercise;    Bovine colostrum;   

Others  :  1165159
DOI  :  10.1186/1756-0500-5-649

 received in 2012-07-12, accepted in 2012-11-19,  发布年份 2012

【 摘 要 】

Background

This study examined the effects of bovine colostrum on exercise –induced modulation of antioxidant parameters in skeletal muscle in mice. Adult male BALB/c mice were randomly divided into four groups (control, colostrum alone, exercise and exercise with colostrum) and each group had three subgroups (day 0, 21 and 42). Colostrum groups of mice were given a daily oral supplement of 50 mg/kg body weight of bovine colostrum and the exercise group of mice were made to exercise on the treadmill for 30 minutes per day. Total antioxidants, lipid hydroperoxides, xanthine oxidase and super oxide dismutase level was assayed from the homogenate of hind limb skeletal muscle.

Results

Exercise—induced a significant oxidative stress in skeletal muscles as evidenced by the elevated lipid hydroperoxides and xanthine oxidase levels. There was a significant decrease in skeletal muscle total antioxidants and superoxide dismutase levels. Daily colostrum supplement significantly reduced the lipid hydroperoxides and xanthine oxidase enzyme level and increased the total antioxidant levels in the leg muscle.

Conclusion

Thus, the findings of this study showed that daily bovine colostrum supplementation was beneficial to skeletal muscle to reduce the oxidant-induced damage during muscular exercise.

【 授权许可】

   
2012 Appukutty et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Choi HS, Jung KH, Lee SC, Yim SV, Chung JH, Kim YW, Jeon WK, Hong HP, Ko YG, Kim CH, Jang KH, Kang SA: Bovine colostrum prevents bacterial translocation in an intestinal ischemia/reperfusion-injured rat model. J Med Food 2009, 12:37-46.
• [2]Greenberg PD, Cello JP: Treatment of severe diarrhoea caused by Cryptosporidium parvum with oral bovine immunoglobulin concentrate in patients with AIDS. J Acq Immun Def Synd 1996, 13:348-354.
• [3]Struff WG, Sprotte G: Bovine colostrum as a biologic in clinical medicine: review. Int J Clin Pharmacol Therapeut 2007, 45:193-202.
• [4]Topham D, Debra MS: Bovine colostrum: a novel protein source in the consumer marketplace. Nutrition M.D 2006, 32:6.
• [5]Brinkworth GD, Buckely JD, Bourden PC, Gulbin GP, David A: Oral bovine colostrum supplementation enhances buffer capacity but not rowing performance in elite female rowers. Int J Sport Nutr Exer Metabol 2002, 12:349-363.
• [6]Mero A, Kahkonen J, Nykanen T, Parviainen T, Jokinen I, Takala T, Nikula T, Rasi S, Leppaluoto J: IGF-I, IgA, and IgG responses to bovine colostrum supplementation during training. J Appl Physiol 2002, 93:732-739.
• [7]Crooks CV, Wall CR, Cross ML, Rutherfurd-Markwic KJ: The effect of bovine colostrum supplementation on salivary IgA in distance runners. Int J Sport Nutr Exer Metabol 2006, 16:47-64.
• [8]Shing CM, Jenkins DG, Stevenson L, Coombes JS: The influence of bovine colostrum supplementation on exercise performance in highly trained cyclists. Br J Sports Med 2006, 40:797-801.
• [9]Mero A, Mikkulainen H, Riski J, Pakkanen R, Aalto J, Takala T: Effects of bovine colostrum supplementation on serum IGF-1, IgG, hormone and saliva IgA during training. J Appl Physiol 1997, 83:1144-1151.
• [10]Antonio J, Sanders MS, Van Gammeren D: The effects of bovine colostrum supplementation on body composition and exercise performance in active men and women. Nutrition 2001, 17:243-247.
• [11]Hofman Z, Smeets R, Verlaan G, Lugt R, Verstappen PA: The effect of bovine colostrum supplementation on exercise performance in elite field hockey players. Int J Sport Nutr Exer Metabol 2002, 12:461-469.
• [12]Jackson MJ, Papa S, Bolanos J, Bruckdorfer R, Carlsen H, Elliott RM, et al.: Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function. Mol Aspects Med 2002, 23:209-285.
• [13]Chang CK, Huang HY, Tseng HF, Hsuuw YD, Tso TK: Interaction of vitamin E and exercise training on oxidative stress and antioxidant enzyme activities in rat skeletal muscles. J Nutr Biochem 2007, 18:39-45.
• [14]Arent SM, Senso M, Golem DL, McKeever KH: The effects of theaflavin-enriched black tea extract on muscle soreness, oxidative stress, inflammation, and endocrine responses to acute anaerobic interval training: a randomized, double-blind, crossover study. J Int Soc Sports Nutr 2010, 23:11.
• [15]Margaritis I, Rousseau AS: Does physical exercise modify antioxidant requirements? Nutr Res Rev 2008, 21:3-12.
• [16]Trappe S, Williamson D, Godard M, Porter D, Rowden G, Costill D: Effect of resistance training on single muscle fiber contractile function in older men. J Appl Physiol 2000, 89:143-152.
• [17]Balagopal P, Schimke JC, Ades P, Adey D, Nair KS: Age effect on transcript levels and synthesis rate of muscle MHC and response to resistance exercise. Am J Physiol 2001, 280:E203-E208.
• [18]Fehrenbach E, Northoff H: Free radicals, exercise, apoptosis, and heat shock proteins. Exer Immunol Rev 2001, 7:66-89.
• [19]Ji LL: Exercise-induced modulation of antioxidant defense. Ann N Y Acad Sci 2002, 959:82-92.
• [20]Reddy KV, Kumar TC, Prasad M, Reddanna P: Pulmonary lipid peroxidation and antioxidant defences during exhaustive physical exercise: the role of vitamin E and selenium. Nutrition 1998, 14:448-451.
• [21]Ji LL, Dillon D, Wu E: Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. Am J Physiol 1990, 258:R918-R923.
• [22]Holloszy JO: Longevity of exercising male rats: effect of an antioxidant supplemented diet. Mech Ageing Dev 1998, 100:211-219.
• [23]Gomez-Cabrera MC, Domenech E, Romagnoli M, Arduini A, Borras C, Pallardo FV, Sastre J, Viña J: Oral administration of vitamin C decreases muscle mitochondrial biogenesis and hampers training-induced adaptations in endurance performance. Am J Clin Nutr 2008, 87:142-149.
• [24]Peternelj TT, Coombes JS: Antioxidant supplementation during exercise training: beneficial or detrimental? Sports Med 2011, 41:1043-1069.
• [25]Ristow M, Zarse K, Oberbach A, Klöting N, Birringer M, Kiehntopf M, Stumvoll M, Kahn CR, Blüher M: Antioxidants prevent health-promoting effects of physical exercise in humans. Proc Natl Acad Sci USA 2009, 106:8665-8670.
• [26]Mattson MP: Hormesis defined. Ageing Res Rev 2008, 7:1-7.
• [27]Reid MB: Invited review: redox modulation of skeletal muscle contraction: what we know and what we don’t. J Appl Physiol 2001, 90:724-731.
• [28]Laughlin MH, Simpson T, Sexton WL, Brown OR, Smith JK, Korthuis RJ: Skeletal muscle oxidative capacity, antioxidant enzymes, and exercise training. J Appl Physiol 1990, 68:2337-2343.
• [29]Ji LL, Wu E, Thomas DP: Effect of exercise training on antioxidant and metabolic functions in senescent rat skeletal muscle. Gerontology 1991, 37:317-325.
• [30]Chiang SH, Chang CY: Antioxidant properties of caseins and whey proteins from colostrums. J Food Drug Anal 2005, 13:57-63.
• [31]Przybylska J, Albera E, Kankofer M: Antioxidants in bovine colostrum. Repro Domest Anim 2007, 42:402-409.
• [32]Albera E, Kankofer M: Antioxidants in colostrum and milk of sows and cows. Repro Domest Anim 2009, 44:606-611.
• [33]Alberti-Fidanza A, Burini G, Perriello G: Total antioxidant capacity of colostrum and transitional and mature human milk. J Matern Fetal Neonatal Med 2002, 11:275-279.
• [34]Bejma J, Ji LL: Aging and acute exercise enhance free radical generation in rat skeletal muscle. J Appl Physiol 1999, 87:465-470.
• [35]Witt EH, Reznick AZ, Viguie CA, Starke-Reed P, Packer L: Exercise, oxidative damage and effects of antioxidant manipulation. J Nutr 1992, 122:766-773.
• [36]Hagiwara K, Kataoka S, Yamanaka H, Kirisawa R, Iwai H: Detection of cytokines in bovine colostrum. Vet Immunol Immunopathol 2000, 76:183-190.
• [37]Zarban A, Taheri F, Chahkandi T, Sharifzadeh G, Khorashadizadeh M: Antioxidant and radical scavenging activity of human colostrum, transitional and mature milk. J Clin Biochem Nutr 2009, 45:150-154.