Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease

BMC Immunology

Bodo Grimbacher³  Frank M Ruemmele²  Wolfgang Kreisel¹  Karin R Engelhardt³  Jennifer Wanders³  Erik-Oliver Glocker⁵  Natalie Frede⁴ 

[1] Department of Gastroenterology, Hepatology and Endocrinology, University Hospital Freiburg, Freiburg, Germany;Université Paris-Descartes, Sorbonne, Paris Cité. INSERM U989, Assistance Publique-Hopitaux de Paris, Hôpital Necker Enfants Malades, Service de Gastroentérologie pédiatrique, Paris, France;Department of Immunology, Division of Infection and Immunity, University College London, Royal Free Hospital, London, UK;Centre of Chronic Immunodeficiency, University Medical Centre Freiburg, Engesser Straße 4, 79108 Freiburg, Germany;Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany

关键词: IL-10 receptor;    Interleukin-10;    Autoimmunity;    Autoantibodies;    Inflammatory bowel disease;   

Others  :  1077723
DOI  :  10.1186/1471-2172-15-10

 received in 2013-07-03, accepted in 2014-02-06,  发布年份 2014

【 摘 要 】

Background

Inflammatory bowel disease constitutes a heterogeneous group of conditions, whose aetiology is only partly understood. The prevailing hypothesis on its pathogenesis is that IBD is the result of an inadequate immune response to the resident bacterial flora of the intestine. An autoimmune background, however, has been discussed since the 1950s. Lately, it has been shown that failures in interleukin-10 (IL-10) signalling due to IL-10- and IL-10 receptor (IL-10R) mutations result in IBD. Our study aimed at investigating the existence of inhibitory autoantibodies against IL-10 and IL-10R in IBD patients capable of down-modulating IL-10 signalling thereby mimicking IL-10 or IL-10R deficiency.

Results

Thirteen IBD patients had IgG autoantibodies against IL-10, IL-10RA and/or IL-10RB, and three patients had IgA autoantibodies against IL-10. However, the absolute OD values of the serum antibodies measured by ELISA were low, there was overall no significant difference between patients and controls, and positive sera had no neutralizing activity.

Conclusion

No evidence for an involvement of autoantibodies against IL-10 or IL-10R in the pathogenesis of inflammatory bowel disease could be established.

【 授权许可】

   
2014 Frede et al.; licensee BioMed Central Ltd.

【 图 表 】

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