Paeonolum protects against MPP+-induced neurotoxicity in zebrafish and PC12 cells

BMC Complementary and Alternative Medicine

Xiao-Li Yao⁴  Zhong Pei⁴  Fei-Wen Huang⁴  Bai-Xuan He⁴  Lei Shi²  Cheng-Hui Ye³  Feng-Juan Su⁴  Qi Wu⁴  Yue-Hao Lin¹  Xi-Lin Lu⁴ 

[1] Department of Clinical Laboratory, Cancer Center of Sun Yat-sen University, Guangzhou 510060, China;Neurosurgery Intensive Care Unit, Department of Critical Care Medicine, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Department of Geriatrics, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China

关键词: ROS;    Total GSH;    Zebrafish;    Mitochondrial;    MPP+;    Paeonolum;   

Others  :  1178923
DOI  :  10.1186/s12906-015-0661-0

 received in 2014-12-03, accepted in 2015-04-22,  发布年份 2015

【 摘 要 】

Background

Parkinson’s disease (PD) is the second most common neurodegenerative disease, affecting 2% of the population aged over 65 years old. Mitochondrial defects and oxidative stress actively participate in degeneration of dopaminergic (DA) neurons in PD. Paeonolum, a main component isolated from Moutan cortex, has potent antioxidant ability. Here, we have examined the effects of paeonolum against MPP⁺-induced neurotoxicity in zebrafish and PC12 cells.

Methods

The overall viability and neurodegeneration of DA neurons was assessed in ETvmat2:green fluorescent protein (GFP) transgenic zebrafish, in which most monoaminergic neurons are labeled by GFP. Damage to PC12 cells was measured using a cell viability assay and assessment of nuclear morphology. Intracellular reactive oxygen species (ROS) and the level of total GSH were assessed. The mitochondrial cell death pathway including mitochondrial membrane potential, cytochrome C release and caspase-3 activity were also examined in PC12 cells.

Results

Paeonolum protected against MPP⁺-induced DA neurodegeneration and locomotor dysfunction in zebrafish in a concentration-dependent manner. Similar neuroprotection was replicated in the PC12 cellular model of MPP⁺ toxicity. Paeonolum attenuated MPP⁺-induced intracellular ROS accumulation and restored the level of total GSH in PC12 cells. Furthermore, paeonolum significantly inhibited the mitochondrial cell death pathway induced by MPP⁺.

Conclusions

Collectively, the present study demonstrates that paeonolum protects zebrafish and PC12 cells against MPP⁺-induced neurotoxicity.

【 授权许可】

   
2015 Lu et al.; licensee BioMed Central.

【 图 表 】

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