Anti-thrombotic effects of α-linolenic acid isolated from Zanthoxylum bungeanum Maxim seeds

BMC Complementary and Alternative Medicine

Siwang Wang¹  Yanhua Xie¹  Wei Cao¹  Xuanxuan Zhou¹  Weidong Cao²  Qian Yang¹ 

[1] Department of Materia Medica, School of Pharmacology, Fourth Military Medical University, Xi’an, Shaanxi, China;Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, China

关键词: Unsaturated fatty acids;    Linoleic acid;    Zanthoxylum bungeanum Maxim seeds;    Anti-thrombotic;    Alpha-linolenic acid;   

Others  :  1086630
DOI  :  10.1186/1472-6882-14-348

 received in 2014-05-28, accepted in 2014-09-16,  发布年份 2014

【 摘 要 】

Background

The current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo.

Methods

The seeds were crushed and subsequently subjected to saponification, acid hydrolysis, gradient freezing, urea inclusion and complexation of silver nitrate to obtain the unsaturated fatty acids. The chemical characteristics of isolated ALA were validated by ¹HNMR, ¹³CNMR and mass spectrometry, and then the anti-thrombotic effect of ALA and its mixture with linoleic acid (1:1) were evaluated in the following experiments.

Results

The alpha-linolenic acid was isolated and purified from Jiaomu through our newly established methods. ALA and its mixture with linoleic acid can prolong the hemorrhage and coagulation time as well as enhanced the survival rate of mice subjected to collagen-adrenaline induced thrombosis. In addition, the thrombosis on A-V bypass and platelet aggregation of rats will be reduced after treated with ALA or its mixture, and the expression level of Akt and PI3K protein decreased 26% and 31%, respectively.

Conclusions

We designed and optimized a very simple and high-yield procedure to isolate ALA and linoleic acid mixture from seeds of Zanthoxylum bungeanum Maxim and demonstrated that such mixture can obtain a good anti-thrombotic effect through the modulation of PI3K/Akt signaling.

【 授权许可】

   
2014 Yang et al.; licensee BioMed Central Ltd.

【 图 表 】

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