Anti-thrombotic effects of α-linolenic acid isolated from Zanthoxylum bungeanum Maxim seeds

BMC Complementary and Alternative Medicine

Research Article

Xuanxuan Zhou¹  Siwang Wang¹  Wei Cao¹  Yanhua Xie¹  Qian Yang¹  Weidong Cao² 

[1] Department of Materia Medica, School of Pharmacology, Fourth Military Medical University, Xi’an, Shaanxi, China;Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, China;

关键词: Alpha-linolenic acid;    Anti-thrombotic;    Zanthoxylum bungeanum Maxim seeds;    Linoleic acid;    Unsaturated fatty acids;   

DOI  :  10.1186/1472-6882-14-348

 received in 2014-05-28, accepted in 2014-09-16,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo.MethodsThe seeds were crushed and subsequently subjected to saponification, acid hydrolysis, gradient freezing, urea inclusion and complexation of silver nitrate to obtain the unsaturated fatty acids. The chemical characteristics of isolated ALA were validated by 1HNMR, 13CNMR and mass spectrometry, and then the anti-thrombotic effect of ALA and its mixture with linoleic acid (1:1) were evaluated in the following experiments.ResultsThe alpha-linolenic acid was isolated and purified from Jiaomu through our newly established methods. ALA and its mixture with linoleic acid can prolong the hemorrhage and coagulation time as well as enhanced the survival rate of mice subjected to collagen-adrenaline induced thrombosis. In addition, the thrombosis on A-V bypass and platelet aggregation of rats will be reduced after treated with ALA or its mixture, and the expression level of Akt and PI3K protein decreased 26% and 31%, respectively.ConclusionsWe designed and optimized a very simple and high-yield procedure to isolate ALA and linoleic acid mixture from seeds of Zanthoxylum bungeanum Maxim and demonstrated that such mixture can obtain a good anti-thrombotic effect through the modulation of PI3K/Akt signaling.

【 授权许可】

Unknown   
© Yang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.