Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

BMC Complementary and Alternative Medicine

Martin Steven Myer¹  Jacobus Nicolaas Eloff⁵  Jana Olivier¹  Gerda Fouche⁴  Paul Anton Steenkamp³  Victor Patrick Bagla²  Lyndy Joy McGaw⁵ 

[1] Department of Geography, Geoinformatics and Meteorology, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa;Present address: Department of Biochemistry, Microbiology and Biotechnology, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa;Department of Biochemistry, University of Johannesburg, Auckland Park 2006, South Africa;Biosciences, Council for Scientific and Industrial Research, PO Box 395, Pretoria, Gauteng 0001, South Africa;Department of Paraclinical Sciences, Phytomedicine Programme, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa

关键词: Zulu tea;    HPLC;    Bioautography;    Athrixia phylicoides;    Asteraceae;    Antifungal;    Antibacterial;   

Others  :  1220440
DOI  :  10.1186/1472-6882-13-356

 received in 2012-12-31, accepted in 2013-12-10,  发布年份 2013

【 摘 要 】

Background

Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential.

Methods

Extracts of the dried ground aerial parts were prepared using organic solvents (diethyl ether, dichloromethane/methanol, ethyl acetate and ethanol) and water. These extracts were subjected to HPLC, TLC and bioautography analysis with the aim of linking a range of peaks visualized in HPLC chromatography profiles to antibacterial and antifungal activity of the same extracts.

Results

HPLC revealed a group of compounds extracted by more than one solvent. Compounds identified include inositol, caffeic acid, quercetin, kaempferol, apigenin, hymenoxin and oleanolic acid. The organic extracts displayed similar TLC profiles, and bioautography indicated approximately five antibacterial compounds, but only two antifungal compounds in these extracts. Bioautography indicated that cold water extracted the least antimicrobial compounds.

Conclusions

Several previously unknown compounds were identified in Athrixia phylicoides extracts, and bioautography indicated a number of antibacterial and antifungal compounds. There were notable differences in chemical composition and bioactivity between the organic and aqueous extracts. Further research is necessary to fully characterize the active components of the extracts.

【 授权许可】

   
2013 McGaw et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Leistner OA: Seed plants of southern Africa: families and genera. Strelitzia 10. Pretoria: National Botanical Institute; 2000.
• [2]Pooley E: A field guide to wild flowers of KwaZulu-Natal and the Eastern region. Durban: Natal Flora Publications Trust; 1998:442.
• [3]Mudau FN, Araya HT, du Toit ES, Soundy P, Olivier J: Bush tea (Athrixia phylicoides DC.) as an alternative herbal and medicinal plant in southern Africa: opportunity for commercialization. Med Aromat Plant Sci Biotechnol 2007, 1:70-73.
• [4]Rampedi I, Olivier J: The use and potential commercial development of Athrixia phylicoides. Acta Academica 2005, 37:165-183.
• [5]de Beer D, Joubert E: Antioxidant potential and phenolic composition of bush tea (Athrixia phylicoides) extracts. In Abstracts of Indigenous Plant Use Forum. Johannesburg, South Africa; 2007:30.
• [6]Mavundza EJ, Tshikalange TE, Mudau FN, Meyer JJM: Evaluation of antibacterial and antioxidant activities in ethanol extract of wild bush tea (Athrixia phylicoides (DC.)). Med Aromat Plant Sci Biotechnol 2007, 1:240-242.
• [7]Mavundza EJ, Tshikalange TE, Lall N, Mudau FN, Hussein AA: Antioxidant activity and cytotoxicity of three flavonoids from Athrixia phylicoides ethanol extract. S Afr J Bot 2010, 76:398.
• [8]McGaw LJ, Steenkamp V, Eloff JN: Evaluation of Athrixia bush tea for cytotoxicity, antioxidant activity, caffeine content and presence of pyrrolizidine alkaloids. J Ethnopharmacol 2007, 110:16-22.
• [9]Padayachee K: The phytochemistry and biological activities of Athrixia phylicoides. Johannesburg, South Africa: MSc Med. Thesis. University of the Witwatersrand; 2010.
• [10]Hutchings A, Scott AH, Lewis G, Cunningham A: Zulu Medicinal Plants: An Inventory. Pietermaritzburg, South Africa: University of Natal Press; 1996.
• [11]Mabogo DEN: The ethnobotany of the Vhavenda. Pretoria, South Africa: MSc Thesis, University of Pretoria; 1990.
• [12]Watt JM, Breyer-Brandwijk MG: Medicinal and Poisonous Plants of Southern and Eastern Africa. 2nd edition. Edinburgh: Livingstone; 1962.
• [13]Rakuambo ZJ: Indigenous knowledge of bush tea (Athrixia phylicoides) and effect of fertigation frequency and growing medium on plant growth. Pretoria, South Africa: M.Inst. Agrar Thesis, University of Pretoria; 2007.
• [14]van Wyk B-E, Gericke N: People’s Plants. Pretoria, South Africa: Briza; 2000.
• [15]de Beer D, Joubert E, Malherbe CJ, Brand DJ: Use of countercurrent chromatography during isolation of 6-hydroxyluteolin-7-O-B-glucoside, a major antioxidant of Athrixia phylicoides. J Chromatogr A 2011, 1218:6179-6186.
• [16]Mogotlane ID, Mudau FN, Mashela PW, Soundy P: Seasonal responses of total antioxidant contents in cultivated Bush Tea (Athrixia phylicoides L.) leaves to fertilizer rates. Medicinal Aromatic Plant Sci Biotech 2007, 1:77-79.
• [17]Chellan N, Muller CJF, de Beer D, Joubert E, Page BJ, Louw J: An in vitro assessment of the effect of Athrixia phylicoides DC. aqueous extract on glucose metabolism. Phytomedicine 2012, 19:730-736.
• [18]Bohlmann F, Zdero C: Neue norkauren- und thymol-derivate aus Athrixiaarten. Phytochem 1977, 16:1773-1776.
• [19]Mashimbye MJ, Mudau FN, Soundy P, van Ree T: A new flavonol from Athrixia phylicoides (bush tea). S Afr J Chem 2006, 59:1-2.
• [20]Reichelt KV, Hoffmann-Luecke P, Hartmann B, Weber B, Ley JP, Krammer GE, Swanepoel KM, Engel K-H: Phytochemical characterization of South African bush tea (Athrixia phylicoides DC.). S Afr J Bot 2012, 83:1-8.
• [21]Mudau FN: Growth, development and chemical composition of bush tea (Athrixia phylicoides L.) as affected by seasonal nitrogen, phosphorus and potassium nutrition. Pretoria, South Africa: PhD Thesis, University of Pretoria; 2006.
• [22]Mudau FN, Soundy P, du Toit ES, Olivier J: Variation in polyphenolic content of Athrixia phylicoides (L.) (bush tea) leaves with season and nitrogen application. S Afr J Bot 2006, 72:398-402.
• [23]Chabeli PM, Mudau FN, Mashela PW, Soundy P: Effects of nitrogen, phosphorus and potassium nutrition on seasonal tannin content of bush tea (Athrixia phylicoides DC.). S Afr J Plant & Soil 2013, 25:79-83.
• [24]Mudau FN, Soundy P, du Toit ES: Effects of nitrogen, phosphorus, and potassium nutrition on total polyphenol content of bush tea (Athrixia phylicoides L.) leaves in a shaded nursery environment. Hortscience 2007, 42:334-338.
• [25]Mudau FN, Soundy P, du Toit ES: Nitrogen, phosphorus, and potassium nutrition increases growth and total polyphenol concentrations of bush tea in a shaded nursery environment. Horttechnology 2007, 17:107-110.
• [26]Chellan N, de Beer D, Muller C, Joubert E, Louw J: A toxicological assessment of Athrixia phylicoides aqueous extract following sub-chronic ingestion in a rat model. Human Exp Toxicol 2008, 27:819-825.
• [27]Hamburger MO, Cordell GA: A direct bioautographic TLC assay for compounds possessing antibacterial activity. J Nat Prod 1987, 50:19-22.
• [28]Rahalison L, Hamburger M, Hostettmann K, Monod M, Frenk E: A bioautographic agar overlay method for the detection of antifungal compounds from higher plants. Phytochem Anal 1991, 2:199-203.
• [29]Cos P, Vlietinck AJ, Vanden Berghe D, Maes L: Anti-infective potential of natural products: how to develop a stronger in vitro ‘proof-of-concept’. J Ethnopharmacol 2006, 106:290-302.
• [30]Begue WJ, Kline RM: The use of tetrazolium salts in bioautographic procedures. J Chromatogr 1972, 64:182-184.
• [31]Masoko P, Eloff JN: The diversity of antifungal compounds of six South African Terminalia species (Combretaceae) determined by bioautography. Afr J Biotechnol 2005, 4:1425-1431.
• [32]Joubert E, Gelderblom WCA, Louw A, de Beer D: South African herbal teas: Aspalathus linearis, Cyclopia spp. and Athrixia phylicoides—A review. J Ethnopharmacol 2008, 119:376-412.
• [33]Kamatou GPP, Viljoen AM, Gono-Bwalya AB, van Zyl RL, van Vuuren SF, Lourens ACU, Başer KHC, Demirci B, Lindsey KL, van Staden J, Steenkamp P: The in vitro pharmacological activities and a chemical investigation of three South African Salvia species. J Ethnopharmacol 2005, 102:382-390.
• [34]Kotze M, Eloff JN: Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae). S Afr J Bot 2002, 68:62-67.
• [35]Wagner H, Bladt S: Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd edition. Berlin: Springer-Verlag; 1996.
• [36]Hagerman AE: Vanillin Assay. http://www.users.muohio.edu/hagermae/Vanillin%20Assay.pdf webcite (accessed 4 July 2013)
• [37]Devi PU, Ganasoundari A: Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum. Indian J Exp Biol 1995, 33:205-208.
• [38]Aina SA, Banjo AD, Lawal OA, Okoh HI, Aina OO, Dedeke GA: The toxicity of extracts of Tetrapleura tetraptera (Aridan), Delonix regia (Flame of the Forest) and Raphia vinifera (Raffia Palm) on the larvae of Anopheles gambiae. Acad J Entomol 2009, 2:67-70.
• [39]Tarkang PA, Agbor GA, Armelle TD, Ramthe TLR, David K, Ngadena YSM: Acute and chronic toxicity studies of the aqueous and ethanol leaf extracts of Carica papaya Linn in Wistar rats. J Nat Prod Plant Resour 2012, 2:617-627.
• [40]Harwood M, Danielewska-Nikiel B, Borzelleca JF, Flamm GW, Williams GM, Lines TC: A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties. Food Chem Toxicol 2007, 45:2179-2205.
• [41]Chen AY, Chen YC: A review of the dietary flavonoid, kaempferol on human health and cancer chemoprevention. Food Chem 2013, 138:2099-2107.
• [42]Tsuji PA, Walle T: Cytotoxic effects of the dietary flavones chrysin and apigenin in a normal trout liver cell line. Chem Biol Interact 2008, 171:37-44.
• [43]Liu J: Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol 1995, 49:57-68.
• [44]The Merck Veterinary Manual for Veterinary Professionals http://www.merckmanuals.com/vet/search.html?qt=hymenoxin&start=1&context=%2Fvet webcite (accessed 27 September 2013)
• [45]The Food and Environment Research Agencyhttps://secure.fera.defra.gov.uk/treechemicals/review/metabolites.cfm webcite (accessed 27 September 2013)
• [46]Scheepers S: Anti-microbial activity of rooibos tea (Aspalathus linearis) on food spoilage organisms and potential pathogens. Stellenbosch, South Africa: M.Sc. (Food Science) Thesis. Stellenbosch University; 2001.