RNA interference machinery-mediated gene regulation in mouse adult neural stem cells

BMC Neuroscience

D. Chichung Lie²  Silvia Cappello¹  Frederick Rehfeld⁵  Rossella Di Giaimo⁴  Filippo M. Cernilogar³ 

[1] Developmental Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany;Institute of Biochemistry, Emil Fischer Center, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany;Biomedical Center, Ludwig Maximilian University, Großhaderner Strasse 9, Planegg-Martinsried, 82152, Germany;Department of Biology, University of Naples Federico II, Naples, Italy;Institute of Cell Biology and Neurobiology, Charité University, Berlin, Germany

关键词: Adult neurogenesis;    miRNA-128;    Doublecortin;    Argonaute;    Dicer;    RNAi;   

Others  :  1230647
DOI  :  10.1186/s12868-015-0198-7

 received in 2014-10-11, accepted in 2015-09-08,  发布年份 2015

【 摘 要 】

Background

Neurogenesis in the brain of adult mammals occurs throughout life in two locations: the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus in the hippocampus. RNA interference mechanisms have emerged as critical regulators of neuronal differentiation. However, to date, little is known about its function in adult neurogenesis.

Results

Here we show that the RNA interference machinery regulates Doublecortin levels and is associated with chromatin in differentiating adult neural progenitors. Deletion of Dicer causes abnormal higher levels of Doublecortin. The microRNA pathway plays an important role in Doublecortin regulation. In particular miRNA-128 overexpression can reduce Doublecortin levels in differentiating adult neural progenitors.

Conclusions

We conclude that the RNA interference components play an important role, even through chromatin association, in regulating neuron-specific gene expression programs.

【 授权许可】

   
2015 Cernilogar et al.

【 图 表 】

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