Single Molecule FRET Studies on Argonaute protein

Argonaute;RISC;Single Molecule FRET;단일분자 FRET;571

자연과학대학 생물물리 및 화학생물학과 ;

University:서울대학교 대학원

关键词: Argonaute;    RISC;    Single Molecule FRET;    단일분자 FRET;    571;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/131526/1/000000020916.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

RNA silencing processes have a variety of functions, including gene expression control. These processes, such as the RNA interference and microRNA pathways use small RNAs that play crucial roles in regulating gene expression. However, small RNAs cannot work alone. They should, with Argonaute proteins and other cofactors, form RISCs (RNA-induced silencing complex) to do their gene silencing functions. Although overall the mechanism of RNA silencing processes is well understood, many molecular details of these pathways are not. Here, by using single-molecule fluorescence resonance transfer (FRET) assays and human Argonaute 2 (hAgo2), we monitor the molecular details and kinetics of RNA silencing pathways.