RNA interference machinery-mediated gene regulation in mouse adult neural stem cells

BMC Neuroscience

Research Article

Silvia Cappello¹  Rossella Di Giaimo²  Filippo M. Cernilogar³  D. Chichung Lie⁴  Frederick Rehfeld⁵ 

[1] Developmental Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany;Institute for Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany;Department of Biology, University of Naples Federico II, Naples, Italy;Research Group Adult Neurogenesis and Neural Stem Cells, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany;Biomedical Center, Ludwig Maximilian University, Großhaderner Strasse 9, 82152, Planegg-Martinsried, Germany;Research Group Adult Neurogenesis and Neural Stem Cells, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany;Institute of Biochemistry, Emil Fischer Center, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany;Research Group Adult Neurogenesis and Neural Stem Cells, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich-Neuherberg, Germany;Institute of Cell Biology and Neurobiology, Charité University, Berlin, Germany;

关键词: RNAi;    Dicer;    Argonaute;    Doublecortin;    miRNA-128;    Adult neurogenesis;   

DOI  :  10.1186/s12868-015-0198-7

 received in 2014-10-11, accepted in 2015-09-08,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundNeurogenesis in the brain of adult mammals occurs throughout life in two locations: the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus in the hippocampus. RNA interference mechanisms have emerged as critical regulators of neuronal differentiation. However, to date, little is known about its function in adult neurogenesis.ResultsHere we show that the RNA interference machinery regulates Doublecortin levels and is associated with chromatin in differentiating adult neural progenitors. Deletion of Dicer causes abnormal higher levels of Doublecortin. The microRNA pathway plays an important role in Doublecortin regulation. In particular miRNA-128 overexpression can reduce Doublecortin levels in differentiating adult neural progenitors.ConclusionsWe conclude that the RNA interference components play an important role, even through chromatin association, in regulating neuron-specific gene expression programs.

【 授权许可】

CC BY   
© Cernilogar et al. 2015