Molecular epidemiology of human sporotrichosis in Venezuela reveals high frequency of Sporothrix globosa

BMC Infectious Diseases

Gustavo A Niño-Vega¹  Mireya Mendoza²  Sabrina Rodríguez-Brito¹  Isabel León-Navarro¹  Emma Camacho¹ 

[1] Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela;Laboratorio de Micología, Instituto de Biomedicina, Caracas, Venezuela

关键词: Venezuela;    American continent;    Phylogenetic analysis;    Phenotypic identification;    Clinical forms;    Sporotrichosis;    Sporothrix globosa;    Sporothrix schenckii species complex;   

Others  :  1135697
DOI  :  10.1186/s12879-015-0839-6

 received in 2014-11-18, accepted in 2015-02-16,  发布年份 2015

【 摘 要 】

Background

Sporotrichosis is a cutaneous and subcutaneous fungal disease of humans and other mammals, known to be caused by the Sporothrix schenckii species complex, which comprises four species of clinical importance: S. brasiliensis, S. globosa, S. luriei, and S. schenckii sensu stricto. Of them, S. globosa and S. schenckii s. str. show global distribution and differences in global frequency as causal agents of the disease. In the Americas, only three species are present: S. schenckii s. str., S. brasiliensis (so far, only reported in Brazil), and S. globosa. In Venezuela, since the first case of sporotrichosis reported in 1935, S. schenckii have been considered its unique etiological agent. In the present work, the presence of more than one species in the country was evaluated.

Methods

By phenotypic key features and molecular phylogeny analyses, we re-examined 30 isolates from diverse Venezuelan regions belonging to the fungi collection of Instituto de Biomedicina, Caracas, Venezuela, and national reference center for skin diseases. All isolates were collected between 1973 and 2013, and maintained in distilled water.

Results

Sporotrichosis in Venezuela is mainly caused by S. schenckii s. str. (70%). However, a significant proportion (30%) of sporotrichosis cases in the country can be attributable to S. globosa. A correlation between intraspecific genotypes and clinical presentation is proposed.

Conclusions

Our data suggest that sporotrichosis various clinical forms might be related to genetic diversity of isolates, and possibly, to diverse virulence profiles previously reported in the S. schenckii species complex. Sporothrix globosa was found to be the causative agent of 30% of sporotrichosis for the Venezuelan cases re-examined, the highest frequency of this species so far reported in the Americas. The high genetic variability presented by S. schenckii s. str. indicates that species distinction based on phenotypic key features could be a challenging and uncertain task; molecular identification should be always employed.

【 授权许可】

   
2015 Camacho et al.; licensee BioMed Central.

【 图 表 】

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【 参考文献 】

• [1]Lopez-Romero E, Reyes-Montes Mdel R, Perez-Torres A, Ruiz-Baca E, Villagomez-Castro JC, Mora-Montes HM, et al.: Sporothrix schenckii complex and sporotrichosis, an emerging health problem. Future Microbiol 2011, 6(1):85-102.
• [2]Rodrigues AM, de Melo Teixeira M, de Hoog GS, Schubach TM, Pereira SA, Fernandes GF, et al.: Phylogenetic analysis reveals a high prevalence of Sporothrix brasiliensis in feline sporotrichosis outbreaks. PLoS Negl Trop Dis 2013, 7(6):e2281.
• [3]Mackinnon JE, Conti-Diaz IA, Gezuele E, Civila E, da Luz S: Isolation of Sporothrix schenckii from nature and considerations on its pathogenicity and ecology. Sabouraudia 1969, 7(1):38-45.
• [4]Kauffman CA: Sporotrichosis. Clin Infect Dis 1999, 29(2):231-6.
• [5]Lopes-Bezerra LM, Schubach A, Costa RO: Sporothrix schenckii and sporotrichosis. An Acad Bras Cienc 2006, 78(2):293-308.
• [6]Romeo O, Scordino F, Criseo G: New insight into molecular phylogeny and epidemiology of Sporothrix schenckii species complex based on calmodulin-encoding gene analysis of Italian isolates. Mycopathologia 2011, 172(3):179-86.
• [7]Dixon DM, Salkin IF, Duncan RA, Hurd NJ, Haines JH, Kemna ME, et al.: Isolation and characterization of Sporothrix schenckii from clinical and environmental sources associated with the largest U.S. epidemic of sporotrichosis. J Clin Microbiol 1991, 29(6):1106-13.
• [8]Marimon R, Gené J, Cano J, Trilles L, Dos Santos Lazera M, Guarro J: Molecular phylogeny of Sporothrix schenckii. J Clin Microbiol 2006, 44(9):3251-6.
• [9]Barros MB, de Almeida Paes R, Schubach AO: Sporothrix schenckii and Sporotrichosis. Clin Microbiol Rev 2011, 24(4):633-54.
• [10]Verma S, Verma GK, Singh G, Kanga A, Shanker V, Singh D, et al.: Sporotrichosis in sub-himalayan India. PLoS Negl Trop Dis 2012, 6(6):e1673.
• [11]Arrillaga-Moncrieff I, Capilla J, Mayayo E, Marimon R, Mariné M, Gené J, et al.: Different virulence levels of the species of Sporothrix in a murine model. Clin Microbiol Infect 2009, 15(7):651-5.
• [12]Fernández-Silva F, Capilla J, Mayayo E, Guarro J: Virulence of Sporothrix luriei in a murine model of disseminated infection. Mycopathologia 2012, 173(4):245-9.
• [13]Fernandes GF, dos Santos PO, Rodrigues AM, Sasaki AA, Burger E, de Camargo ZP: Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species. Virulence 2013, 4(3):241-9.
• [14]Marimon R, Serena C, Gené J, Cano J, Guarro J: In vitro antifungal susceptibilities of five species of Sporothrix. Antimicrob Agents Chemother 2008, 52(2):732-4.
• [15]Rodrigues AM, de Hoog GS, de Cassia Pires D, Brihante RS, Sidrim JJ, Gadelha MF, et al.: Genetic diversity and antifungal susceptibility profiles in causative agents of sporotrichosis. BMC Infect Dis 2014, 14:219. BioMed Central Full Text
• [16]Rodrigues AM, de Hoog S, de Camargo ZP: Emergence of pathogenicity in the Sporothrix schenckii complex. Med Mycol 2013, 51(4):405-12.
• [17]Zhou X, Rodrigues A, Feng P, de Hoog GS: Global ITS diversity in the Sporothrix schenckii complex. Fungal Divers 2014, 66(1):153-65.
• [18]Marimon R, Cano J, Gené J, Sutton DA, Kawasaki M, Guarro J: Sporothrix brasiliensis, S. globosa, and S. mexicana, three new Sporothrix species of clinical interest. J Clin Microbiol 2007, 45(10):3198-206.
• [19]Marimon R, Gené J, Cano J, Guarro J: Sporothrix luriei: a rare fungus from clinical origin. Med Mycol 2008, 46(6):621-5.
• [20]Dias NM, Oliveira MM, Santos C, Zancope-Oliveira RM, Lima N: Sporotrichosis caused by Sporothrix Mexicana, Portugal. Emerg Infect Dis 2011, 17(10):1975-6.
• [21]Morrison AS, Lockhart SR, Bromley JG, Kim JY, Burd EM: An environmental Sporothrix as a cause of corneal ulcer. Medical Mycol Case Reports 2013, 2:88-90.
• [22]Bommer M, Hutter ML, Stilgenbauer S, de Hoog GS, de Beer ZW, Wellinghausen N: Fatal Ophiostoma piceae infection in a patient with acute lymphoblastic leukaemia. J Med Microbiol 2009, 58(Pt 3):381-5.
• [23]Mariat F: Adaptation of Ceratocystis to a parasitic life in animals–aquisition of a pathogenicity comparable to Sporothrix schenckii. Sabouraudia 1971, 9(3):191-205.
• [24]Mata-Essayag S, Delgado A, Colella MT, Landaeta-Nezer ME, Rosello A, Perez de Salazar C, et al.: Epidemiology of sporotrichosis in Venezuela. Int J Dermatol 2013, 52(8):974-80.
• [25]Calcagno AM, Nino-Vega G, San-Blas F, San-Blas G: Geographic discrimination of Paracoccidioides brasiliensis strains by randomly amplified polymorphic DNA analysis. J Clin Microbiol 1998, 36(6):1733-6.
• [26]O’Donnell K: Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia 2000, 92:919-38.
• [27]Zhang J, Madden TL: PowerBLAST: a new network BLAST application for interactive or automated sequence analysis and annotation. Genome Res 1997, 7(6):649-56.
• [28]Duong TA, de Beer ZW, Wingfield BD, Wingfield MJ: Phylogeny and taxonomy of species in the Grosmannia serpens complex. Mycologia 2012, 104(3):715-32.
• [29]Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011, 28(10):2731-9.
• [30]Nei M: Molecular evolutionary genetics. Columbia University Press, New York; 1987.
• [31]Librado P, Rozas J: DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 2009, 25(11):1451-2.
• [32]Oliveira MM, Almeida-Paes R, Muniz MM, Gutierrez-Galhardo MC, Zancope-Oliveira RM: Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil. Mycopathologia 2011, 172(4):257-67.
• [33]Rodrigues AM, de Hoog G, Zhang Y, de Camargo ZP: Emerging sporotrichosis is driven by clonal and recombinant Sporothrix species. Emerg Microbes Infect 2014, 3:e32.
• [34]Madrid H, Cano J, Gené J, Bonifaz A, Toriello C, Guarro J: Sporothrix globosa, a pathogenic fungus with widespread geographical distribution. Rev Iberoam Micol 2009, 26(3):218-22.
• [35]Sasaki AA, Fernandes GF, Rodrigues AM, Lima FM, Marini MM, Dos SFL, et al.: Chromosomal polymorphism in the Sporothrix schenckii complex. PLoS One 2014, 9(1):e86819.
• [36]Yu X, Wan Z, Zhang Z, Li F, Li R, Liu X: Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China. Mycopathologia 2013, 176(1–2):67-74.
• [37]Ottonelli Stopiglia CD, Magagnin CM, Castrillon MR, Mendes SD, Heidrich D, Valente P, et al.: Antifungal susceptibilities and identification of species of the Sporothrix schenckii complex isolated in Brazil. Med Mycol 2014, 52(1):56-64.