Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm

BMC Research Notes

Eric Chabrière¹  Michel Drancourt¹  Gérard Aboudharam¹  Jérome Terras¹  Guillaume Gotthard¹  Hong T T Huynh¹ 

[1] Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France

关键词: Detection;    Plague;    Yersinia pestis;    Surface plasmon resonance imaging;   

Others  :  1231709
DOI  :  10.1186/s13104-015-1236-3

 received in 2014-09-04, accepted in 2015-06-17,  发布年份 2015

【 摘 要 】

Background

Yersinia pestis, causing deadly plague, is classified as a group A bioterrorism bacterium. Some recent DNA-based methods were used for detection of bioterrorism agents.

Results

Y. pestis was used as a model organism to develop an immunosensor based on surface plasmon resonance imaging (SPRi) using monoclonal antibody against Y. pestis F1 antigen. The experimental approach included step-by-step detection of Y. pestis membrane proteins, lysed bacteria, intact bacteria, mock-infected powder and mock-infected clinical specimens. SPRi detected on average 10 ⁶intact Y. pestis organisms in buffer, in mock-infected powder and in a 1:4 mixture with HEL cells.

Conclusions

This study offers the proof-of-concept of the SPRi-based detection of a human pathogen in both environmental and clinical specimens.

【 授权许可】

   
2015 Huynh et al.

【 图 表 】

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【 参考文献 】

• [1]Raoult D, Mouffok N, Bitam I, Piarroux R, Drancourt M: Plague: history and contemporary analysis. J Infect 2013, 66:18-26.
• [2]Neerinckx SB, Peterson AT, Gulinck H, Deckers J, Leirs H: Geographic distribution and ecological niche of plague in sub-Saharan Africa. Int J Health Geogr 2008, 7:54. BioMed Central Full Text
• [3]Bertherat E, Bekhoucha S, Chougrani S, Razik F, Duchemin JB, Houti L, et al.: Plague reappearance in Algeria after 50 years, 2003. Emerg Infect Dis 2007, 13:1459-1462.
• [4]Bitam I, Ayyadurai S, Kernif T, Chetta M, Boulaghman N, Raoult D, et al.: New rural focus of plague, Algeria. Emerg Infect Dis 2010, 16:1639-1640.
• [5]Riedel S: Biological warfare and bioterrorism: a historical review. Proc (Bayl Univ Med Cent) 2004, 17:400-406.
• [6]Bearden SW, Perry RD (2008) Laboratory maintenance and characterization of Yersinia pestis. In: Current protocols in microbiology. Chapter 5. Wiley, New York
• [7]Drancourt M, Aboudharam G, Signoli M, Dutour O, Raoult D: Detection of 400-year-old Yersinia pestis DNA in human dental pulp: an approach to the diagnosis of ancient septicemia. Proc Natl Acad Sci USA 1998, 95:12637-12640.
• [8]Janse I, Hamidjaja RA, Reusken C: Yersinia pestis plasminogen activator gene homolog in rat tissues. Emerg Infect Dis 2013, 19:342-344.
• [9]Bianucci R, Rahalison L, Massa ER, Peluso A, Ferroglio E, Signoli M: Technical note: a rapid diagnostic test detects plague in ancient human remains: an example of the interaction between archeological and biological approaches (southeastern France, 16th–18th centuries). Am J Phys Anthropol 2008, 136:361-367.
• [10]Chanteau S, Rahalison L, Ralafiarisoa L, Foulon J, Ratsitorahina M, Ratsifasoamanana L, et al.: Development and testing of a rapid diagnostic test for bubonic and pneumonic plague. Lancet 2003, 361:211-216.
• [11]Rajerison M, Dartevelle S, Ralafiarisoa LA, Bitam I, Dinh TN, Andrianaivoarimanana V, et al.: Development and evaluation of two simple, rapid immunochromatographic tests for the detection of Yersinia pestis antibodies in humans and reservoirs. PLoS Negl Trop Dis 2009, 3:e421.
• [12]Jeng K, Hardick J, Rothman R, Yang S, Won H, Peterson S, et al.: Reverse transcription-PCR–electrospray ionization mass spectrometry for rapid detection of biothreat and common respiratory pathogens. J Clin Microbiol 2013, 51:3300-3307.
• [13]Schweighardt AJ, Battaglia A, Wallace MM: Detection of anthrax and other pathogens using a unique liquid array technology. J Forensic Sci 2014, 59:15-33.
• [14]Kato K, Ishimuro T, Arima Y, Hirata I, Iwata H: High-throughput immunophenotyping by surface plasmon resonance imaging. Anal Chem 2007, 79:8616-8623.
• [15]Cherif B, Roget A, Villiers CL, Calemczuk R, Leroy V, Marche PN, et al.: Clinically related protein-peptide interactions monitored in real time on novel peptide chips by surface plasmon resonance imaging. Clin Chem 2006, 52:255-262.
• [16]Puttharugsa C, Wangkam T, Huangkamhang N, Gajanandana O, Himananto O, Sutapun B, et al.: Development of surface plasmon resonance imaging for detection of Acidovorax avenae subsp. citrulli (Aac) using specific monoclonal antibody. Biosens Bioelectron 2011, 26:2341-2346.
• [17]Gutierrez-Gallego R, Bosch J, Such-Sanmartin G, Segura J: Surface plasmon resonance immuno assays—a perspective. Growth Horm IGF Res 2009, 19:388-398.
• [18]Lathem WW, Crosby SD, Miller VL, Goldman WE: Progression of primary pneumonic plague: a mouse model of infection, pathology, and bacterial transcriptional activity. Proc Natl Acad Sci USA 2005, 102:17786-17791.
• [19]Mondani L, Roupioz Y, Delannoy S, Fach P, Livache T: Simultaneous enrichment and optical detection of low levels of stressed Escherichia coli O157:H7 in food matrices. J Appl Microbiol 2014, 117:537-546.
• [20]Malou N, Tran TN, Nappez C, Signoli M, Le Forestier C, Castex D, et al.: Immuno-PCR—a new tool for paleomicrobiology: the plague paradigm. PLoS One 2012, 7:e31744.