Candida concentrations determined following concentrated oral rinse culture reflect clinical oral signs

BMC Oral Health

Takayuki Honda³  Eriko Kasuga³  Mitsuo Uchida²  Hiroshi Kurita⁴  Kenji Kurashina¹  Kiyonori Hayashi¹  Takehisa Matsumoto³  Hiroaki Tooyama⁴ 

[1] Department of Dentistry and Oral Surgery, Aizawa Hospital, 2-5-1 Honjo, Matsumoto 390-8510, Japan;Center for Health, Safety and Environmental Management, Shinshu University, 3-1-1 Asahi, Matsumoto 390-8621, Japan;Department of Laboratory Medicine, Shinshu University School of Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan;Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan

关键词: Candida;    Oral candidiasis;    Swab method;    Concentrated oral rinse method;   

Others  :  1234111
DOI  :  10.1186/s12903-015-0138-z

 received in 2015-05-10, accepted in 2015-11-18,  发布年份 2015

【 摘 要 】

Background

Oral candidiasis is an infection caused by a yeast-like fungus called Candida. Various methods can be used to isolate Candida from the oral cavity. However, it is difficult to correctly and satisfactorily diagnose oral candidiasis because currently no microbiological or laboratory standards based on samples from the oral cavity are available. The aim of this study is to establish a reliable laboratory test for diagnosing oral candidiasis.

Methods

Oral swab, rinse and concentrated rinse samples were obtained from 200 consecutive outpatients (103 male patients and 97 female patients; mean age, 47.2 years; age range, 9–89 years). Candida colonies from cultured samples were enumerated to compare the sensitivities and specificities of the above sampling methods, and the associations between Candida detection or concentration and the clinical oral signs were examined.

Results

The mean colony numbers were 263 ± 590 CFU/swab for the swab method, 2894 ± 6705 CFU/100 μL for the rinse method, and 9245 ± 19,030 CFU/100 μL for the concentrated rinse method. The median numbers were 23 CFU/swab for the swab method, 56 CFU/100 μL for the rinse method, and 485 CFU/100 μL for the concentrated rinse method. Candida was detected in the oral cavity of 33.5 % and 52.0 % of the outpatients by the swab method and concentrated rinse, respectively. Candida concentrations determined by the concentrated rinse were closely related to the severity of the clinical oral signs. The positive predictive values of residual root, redness of the oral mucosa, denture, glossalgia, dry mouth, and taste disorder were useful predictors of oral candidiasis.

Conclusions

Concentrated rinse sampling is suitable for evaluating oral candidiasis, and Candida concentrations examined using this method strongly associated with the oral signs associated with Candida infection.

【 授权许可】

   
2015 Tooyama et al.

【 图 表 】

Fig. 1.

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