Antibacterial effect of crude extract and metabolites of Phytolacca americana on pathogens responsible for periodontal inflammatory diseases and dental caries

BMC Complementary and Alternative Medicine

Kwang-Hyun Baek²  Yangseon Kim³  Hyeon-Jeong Kim³  Kyounghee Oh¹  Eun Sil Kim¹  Jayanta Kumar Patra² 

[1] Wildlife Genetic Resources Center, National Institute of Biological Resources, Incheon 404-708, Republic of Korea;School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;Ensoltek Co., Ltd., Techno 10-ro 51, Yuseong-gu, Daejeon 305-510, Republic of Korea

关键词: Streptococcus mutans;    Quercetin;    Porphyromonas gingivalis;    Phytolacca americana;    Kaempferol;   

Others  :  1086665
DOI  :  10.1186/1472-6882-14-343

 received in 2014-06-18, accepted in 2014-09-11,  发布年份 2014

【 摘 要 】

Background

The oral cavity is the store house of different species of microorganisms that are continuously engaged in causing diseases in the mouth. The present study was conducted to evaluate the antibacterial potential of crude extracts of the aerial parts of Phytolacca americana and its natural compounds against two oral pathogens, Porphyromonas gingivalis and Streptococcus mutans, which are primarily responsible for periodontal inflammatory diseases and dental caries, as well as a nonpathogenic Escherichia coli.

Methods

Crude extract and fractions from the aerial parts of P. americana (0.008–1.8 mg/mL) were evaluated for their potential antibacterial activity against two oral disease causing microorganisms by micro-assays. The standard natural compounds present in P. americana, kaempferol, quercetin, quercetin 3-glucoside, isoqueritrin and ferulic acid, were also tested for their antibacterial activity against the pathogens at 1–8 μg/mL.

Results

The crude extract was highly active against P. gingivalis (100% growth inhibition) and moderately active against S. mutans (44% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fraction controlled the growth of P. gingivalis with 91% and 92% growth inhibition at a concentration of 0.2 mg/mL, respectively. Kaempferol exerted antibacterial activity against both the pathogens, whereas quercetin showed potent growth inhibition activity against only S. mutans in a concentration dependent manner.

Conclusion

The crude extract, chloroform fraction, and hexane fraction of P. americana possesses active natural compounds that can inhibit the growth of oral disease causing bacteria. Thus, these extracts have the potential for use in the preparation of toothpaste and other drugs related to various oral diseases.

【 授权许可】

   
2014 Patra et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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