The ColRS signal transduction system responds to the excess of external zinc, iron, manganese, and cadmium

BMC Microbiology

Rita Hõrak¹  Heili Ilves¹  Karl Mumm¹  Kadi Ainsaar¹ 

[1] Institute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, Estonia

关键词: Pseudomonas putida;    Signal perception;    Histidine kinase;    Metal tolerance;    ColRS two-component system;   

Others  :  821401
DOI  :  10.1186/1471-2180-14-162

 received in 2014-02-14, accepted in 2014-06-17,  发布年份 2014

【 摘 要 】

Background

The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by ColS have remained unknown. Here we aimed to analyze the role of the ColRS system in metal tolerance of P. putida and to test whether ColS can respond to metal excess.

Results

We show that the ColRS system is necessary for P. putida to tolerate the excess of iron and zinc, and that it also contributes to manganese and cadmium tolerance. Excess of iron, zinc, manganese or cadmium activates ColRS signaling and as a result modifies the expression of ColR-regulated genes. Our data suggest that the genes in the ColR regulon are functionally redundant, as several loci have to be deleted to observe a significant decrease in metal tolerance. Site-directed mutagenesis of ColS revealed that excess of iron and, surprisingly, also zinc are sensed by a conserved ExxE motif in ColS’s periplasmic domain. While ColS is able to sense different metals, it still discriminates between the two oxidation states of iron, specifically responding to ferric and not ferrous iron. We propose a signal perception model involving a dimeric ColS, where each monomer donates one ExxE motif for metal binding.

Conclusions

Several transition metals are essential for living organisms in certain amounts, but toxic in excess. We show that ColRS is a sensor system which detects and responds to the excess of physiologically important metals such as zinc, iron and manganese. Thus, the ColRS system is an important factor for metal homeostasis and tolerance in P. putida.

【 授权许可】

   
2014 Ainsaar et al.; licensee BioMed Central Ltd.

【 图 表 】

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