In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column

BMC Genomics

Thomas Lufkin²  Shyam Prabhakar¹  Joel Sng³  Siew Lan Lim³  Xing Xing³  Vibhor Kumar¹  Petra Kraus²  Sook Peng Yap³  V Sivakamasundari³  Sumantra Chatterjee³ 

[1] Computational and Mathematical Biology, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore;Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA;Stem Cell and Developmental Biology, 60 Biopolis Street, Singapore 138672, Singapore

关键词: EGFP;    Microarray;    FACS;    ChIP-Seq;    Sox9;    Bapx1;    Expression profiling;    Gene regulatory network;   

Others  :  1127532
DOI  :  10.1186/1471-2164-15-1072

 received in 2014-07-29, accepted in 2014-11-27,  发布年份 2014

【 摘 要 】

Background

Vertebrate organogenesis is a highly complex process involving sequential cascades of transcription factor activation or repression. Interestingly a single developmental control gene can occasionally be essential for the morphogenesis and differentiation of tissues and organs arising from vastly disparate embryological lineages.

Results

Here we elucidated the role of the mammalian homeobox gene Bapx1 during the embryogenesis of five distinct organs at E12.5 - vertebral column, spleen, gut, forelimb and hindlimb - using expression profiling of sorted wildtype and mutant cells combined with genome wide binding site analysis. Furthermore we analyzed the development of the vertebral column at the molecular level by combining transcriptional profiling and genome wide binding data for Bapx1 with similarly generated data sets for Sox9 to assemble a detailed gene regulatory network revealing genes previously not reported to be controlled by either of these two transcription factors.

Conclusions

The gene regulatory network appears to control cell fate decisions and morphogenesis in the vertebral column along with the prevention of premature chondrocyte differentiation thus providing a detailed molecular view of vertebral column development.

【 授权许可】

   
2014 Chatterjee et al.; licensee BioMed Central Ltd.

【 图 表 】

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