Genome-wide mapping of matrix attachment regions in Drosophila melanogaster

BMC Genomics

Rakesh K Mishra¹  Arumugam Srinivasan¹  Rashmi U Pathak¹ 

[1] Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad 500 007, India

关键词: Genome packaging;    Matrix attachment regions;    Nuclear matrix;   

Others  :  1091038
DOI  :  10.1186/1471-2164-15-1022

 received in 2014-06-20, accepted in 2014-11-12,  发布年份 2014

【 摘 要 】

Background

Eukaryotic genome acquires functionality upon proper packaging within the nucleus. This process is facilitated by the structural framework of Nuclear Matrix, a nucleo-proteinaceous meshwork. Matrix Attachment Regions (MARs) in the genome serve as anchoring sites to this framework.

Results

Here we report direct sequencing of the MAR preparation from Drosophila melanogaster embryos and identify >7350 MARs. This amounts to ~2.5% of the fly genome and often coincide with AT rich non-coding regions. We find significant association of MARs with the origins of replication, transcription start sites, paused RNA Polymerase II sites and exons, but not introns, of highly expressed genes. We also identified sequence motifs and repeats that constitute MARs.

Conclusion

Our data reveal the contact points of genome to the nuclear architecture and provide a link between nuclear functions and genomic packaging.

【 授权许可】

   
2014 Pathak et al.; licensee BioMed Central Ltd.

【 图 表 】

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