The effects of vitamins C and B12 on human nasal ciliary beat frequency

BMC Complementary and Alternative Medicine

Luo Zhang¹  Hong Wang¹  Na Meng¹  Jian Jiao¹ 

[1] Key Laboratory of Otolaryngology, Head and Neck Surgery (Ministry of Education), Beijing Institute of Otolaryngology, Beijing, China

关键词: Nasal epithelial cell;    Vitamin B12;    Vitamin C;    Ciliary beat frequency;   

Others  :  1229944
DOI  :  10.1186/1472-6882-13-110

 received in 2012-10-10, accepted in 2013-05-16,  发布年份 2013

【 摘 要 】

Background

This study was designed to investigate the effects of the vitamins C and B12 on the regulation of human nasal ciliary beat frequency (CBF).

Methods

Human nasal mucosa was removed endoscopically and nasal ciliated cell culture was established. Changes of CBF in response to different concentrations of vitamin C or vitamin B12 were quantified by using high-speed (240 frames per second) digital microscopy combined with a beat-by-beat CBF analysis.

Results

At the concentrations of 0.01% and 0.10%, vitamin C induced an initial increase, followed by a gradual decrease of CBF to the baseline level, while 1.00% vitamin C induced a reversible decrease of CBF. Vitamin B12, at the concentrations of 0.01% and 0.10%, did not influence CBF during the 20-min observation period, while a 1.00% vitamin B12 treatment caused a time-dependent but reversible decrease of CBF.

Conclusions

Treatment with vitamin C or vitamin B12 caused a concentration-dependent but reversible decrease of CBF in cultured human nasal epithelial cells. Therefore, it is necessary to choose a concentration that is safe, effective, and non-ciliotoxic when applying these drugs topically in the nasal cavity.

【 授权许可】

   
2013 Jiao et al.; licensee BioMed Central Ltd.

【 图 表 】

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