Transcriptional mechanisms for differential expression of outer membrane cytochrome genes omcA and mtrC in Shewanella oneidensis MR-1

BMC Microbiology

Kazuya Watanabe²  Hideaki Nojiri¹  Atsushi Kouzuma²  Takuya Kasai² 

[1] Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku 113-8657, Tokyo, Japan;School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji 192-0392, Tokyo, Japan

关键词: Shewanella;    Transcriptional regulation;    Outer membrane cytochrome;    Extracellular electron transfer;   

Others  :  1221683
DOI  :  10.1186/s12866-015-0406-8

 received in 2014-11-27, accepted in 2015-03-11,  发布年份 2015

【 摘 要 】

Background

Shewanella oneidensis MR-1 is capable of reducing extracellular electron acceptors, such as metals and electrodes, through the Mtr respiratory pathway, which consists of the outer membrane cytochromes OmcA and MtrC and associated proteins MtrA and MtrB. These proteins are encoded in the mtr gene cluster (omcA-mtrCAB) in the MR-1 chromosome.

Results

Here, we investigated the transcriptional mechanisms for the mtr genes and demonstrated that omcA and mtrC are transcribed from two upstream promoters, P_omcA and P_mtrC, respectively. In vivo transcription and in vitro electrophoretic mobility shift assays revealed that a cAMP receptor protein (CRP) positively regulates the expression of the mtr genes by binding to the upstream regions of P_omcA and P_mtrC. However, the expression of omcA and mtrC was differentially regulated in response to culture conditions; specifically, the expression from P_mtrC was higher under aerobic conditions than that under anaerobic conditions with fumarate as an electron acceptor, whereas expression from P_omcA exhibited the opposite trend. Deletion of the region upstream of the CRP-binding site of P_omcA resulted in a significant increase in promoter activity under aerobic conditions, demonstrating that the deleted region is involved in the negative regulation of P_omcA.

Conclusions

Taken together, the present results indicate that transcription of the mtr genes is regulated by multiple promoters and regulatory systems, including the CRP/cAMP-dependent regulatory system and yet-unidentified negative regulators.

【 授权许可】

   
2015 Kasai et al.; licensee BioMed Central.

【 图 表 】

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