科技报告详细信息
Nanobiogeochemistry of Microbe/Mineral Interactions: A Force Microscopy and Bioinformatics Approach | |
Lower, Steven, K. | |
The Ohio State University | |
关键词: Shewanella; Oxidation; 59 Basic Biological Sciences; Atomic Force Microscopy; Hypothesis; | |
DOI : 10.2172/893095 RP-ID : DOE/ER/15590-1 RP-ID : FG02-04ER15590 RP-ID : 893095 |
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美国|英语 | |
来源: UNT Digital Library | |
【 摘 要 】
Iron-reducing microorganisms, like Shewanella oneidensis, have received a great deal of attention in the literature because of their ability to couple the oxidation of organic contaminants to the reduction of Fe(III) in minerals. The mechanism by which this microorganism transfers electrons to Fe(III) in a mineral’s structure is unknown. We used atomic force microscopy (AFM) to measure forces at the interface between an iron oxide mineral and a living cell of S. oneidensis. A unique force-signature was attributed to outer membrane proteins synthesized for the specific purpose of forming a bond with the surface of an iron oxide. To confirm this hypothesis, we used AFM to measure forces between an iron oxide mineral and each of two outer membrane cytochromes purified from S. oneidensis. There is a strong correlation between the whole cell and pure protein force spectra suggesting that these two cytochromes play a prominent role in the terminal electron transfer to Fe(III) in minerals.【 预 览 】
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893095.pdf | 2750KB | download |