Does phosphorylation of cofilin affect the progression of human bladder cancer?

BMC Cancer

Hong Chung³  Bokyung Kim²  Seung-Hyo Jung²  Kyung-Jong Won²  Xiaowen Jiang²  Chang-Kwon Lee²  So Dug Lim¹  Sang-Kuk Yang³  Ki Hak Song⁴  Hong Sup Kim³ 

[1] Department of Pathology, School of Medicine, Konkuk University, Hwayang-dong, Gwanjin-gu, Seoul, 143-792, Republic of Korea

[2] Department of Physiology, School of Medicine, Konkuk University, 82 Gugwon-daero, Chungju, Chungbuk, 380-704, Republic of Korea

[3] Departments of Urology, School of Medicine, Konkuk University, 82 Gugwon-daero, Chungju, Chungbuk, 380-704, Republic of Korea

[4] Departments of Urology, College of Medicine, Chungnam National University, Chungnam, Republic of Korea

关键词: Urothelial cell carcinoma;    Invasion;    Phosphorylation;    Cofilin;   

Others  :  1079945
DOI  :  10.1186/1471-2407-13-45

 received in 2012-07-26, accepted in 2013-01-28,  发布年份 2013

【 摘 要 】

Background

We determined the differently expressed protein profiles and their functions in bladder cancer tissues with the aim of identifying possible target proteins and underlying molecular mechanisms for taking part in their progression.

Methods

We examined the expression of proteins by proteomic analysis and western blot in normal urothelium, non-muscle-invasive bladder cancers (NMIBCs), and muscle-invasive bladder cancers (MIBCs). The function of cofilin was analyzed using T24 human bladder cancer cells.

Results

The expression levels of 12 proteins were altered between bladder cancers and normal bladder tissues. Of these proteins, 14-3-3σ was upregulated in both NMIBCs and MIBCs compared with controls. On the other hand, myosin regulatory light chain 2, galectin-1, lipid-binding AI, annexin V, transthyretin, CARD-inhibitor of NF-κB-activating ligand, and actin prepeptide were downregulated in cancer samples. Cofilin, an actin-depolymerizing factor, was prominent in both NMIBCs and MIBCs compared with normal bladder tissues. Furthermore, we confirmed that cofilin phosphorylation was more prominent in MIBCs than in NMIBCs using immunoblotting and immunohistochemcal analyses. Epidermal growth factor (EGF) increased the phosphorylation of cofilin and elevated the migration in T24 cells. Knockdown of cofilin expression with small interfering RNA attenuated the T24 cell migration in response to EGF.

Conclusions

These results demonstrate that the increased expression and phosphorylation of cofilin might play a role in the occurrence and invasiveness of bladder cancer. We suspected that changes in cofilin expression may participate in the progression of the bladder cancer.

【 授权许可】

   
2013 Chung et al.; licensee BioMed Central Ltd.

【 图 表 】

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