Prevalence and characterization of Salmonella enterica from the feces of cattle, poultry, swine and hedgehogs in Burkina Faso and their comparison to human Salmonella isolates

BMC Microbiology

Kaisa Haukka¹  Anja Siitonen³  Nicolas Barro²  Alfred S Traoré²  Laura Aulu³  Taru Lienemann³  Assèta Kagambèga² 

[1] Department of Food and Environmental Sciences, Division of Microbiology, University of Helsinki, Helsinki, Finland;Laboratoire de Biologie Moléculaire et d’Epidémiologie et de Surveillance Bactéries et Virus transmis par les Aliments, CRSBAN, Département de Biochimie-Microbiologie, UFR-SVT/Université de Ouagadougou, Ouagadougou, Burkina Faso;Bacteriology Unit, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), Helsinki, Finland

关键词: PFGE;    Genetic relatedness;    Antimicrobial resistance;    Serotypes;    Salmonella;   

Others  :  1142698
DOI  :  10.1186/1471-2180-13-253

 received in 2013-05-11, accepted in 2013-10-31,  发布年份 2013

【 摘 要 】

Background

Production and wild animals are major sources of human salmonellosis and animals raised for food also play an important role in transmission of antimicrobial resistant Salmonella strains to humans. Furthermore, in sub-Saharan Africa non-typhoidal Salmonella serotypes are common bloodstream isolates in febrile patients. Yet, little is known about the environmental reservoirs and predominant modes of transmission of these pathogens. The purpose of this study was to discover potential sources and distribution vehicles of Salmonella by isolating strains from apparently healthy slaughtered food animals and wild hedgehogs and by determining the genetic relatedness between the strains and human isolates. For this purpose, 729 feces samples from apparently healthy slaughtered cattle (n = 304), poultry (n = 350), swine (n = 50) and hedgehogs (n = 25) were examined for the presence of Salmonella enterica in Burkina Faso. The isolates were characterized by serotyping, antimicrobial-susceptibility testing, phage typing, and pulsed-field gel electrophoresis (PFGE) with XbaI and BlnI restriction enzymes.

Results

Of the 729 feces samples, 383 (53%) contained Salmonella, representing a total of 81 different serotypes. Salmonella was present in 52% of the cattle, 55% of the poultry, 16% of the swine and 96% of the hedgehog feces samples. Antimicrobial resistance was detected in 14% of the isolates. S. Typhimurium isolates from poultry and humans (obtained from a previous study) were multiresistant to the same antimicrobials (ampicillin, chloramphenicol, streptomycin, sulfonamides and trimethoprim), had the same phage type DT 56 and were closely related in PFGE. S. Muenster isolates from hedgehogs had similar PFGE patterns as the domestic animals.

Conclusions

Based on our results it seems that production and wild animals can share the same Salmonella serotypes and potentially transmit some of them to humans. As the humans and animals often live in close vicinity in Africa and the hygiene control of the meat retail chain is defective, high Salmonella carriage rates of the animals can pose a major public health risk in Burkina Faso. This underlines the necessity for a joint and coordinated surveillance and monitoring programs for salmonellosis in Africa.

【 授权许可】

   
2013 Kagambèga et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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