Identification of molecular performance from oil palm clones based on SSR markers

Friendly City 4 'From Research to Implementation For Better Sustainability'

地球科学;经济学

Putri, Lollie Agustina P.^1 ; Basyuni, M.^2 ; Bayu, Eva S^1 ; Arvita, D.^1 ; Arifiyanto, D.^3 ; Syahputra, I.^3

Department of Agroecotechnology, Faculty of Agriculture, Universitas Sumatera Utara, Jln. Prof. A. Sofyan no.3, Medan-North-Sumatera

20155, Indonesia^1

Departement of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan

20155, Indonesia^2

PT. Socfin Indonesia, Desa Martebing, Dolok Masihul Kabupaten Serdang Bedagai, Sumatera Utara, Indonesia^3

关键词: DNA profiles;    Genetic relatedness;    Individual tree;    Industrial demands;    PCR products;    Performance analysis;    SSR markers;    Tree stands;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/126/1/012149/pdf
DOI  :  10.1088/1755-1315/126/1/012149

来源: IOP

【 摘 要 】

In Indonesia, the oil palms are an important economic crop, producing food and raw materials for the food, confectionary, cosmetics and oleo-chemical industrial demands of oil palm products. Clonal oil palm offers the potential for greater productivity because it is possible to establish uniform tree stands comprising identical copies (clones) of a limited number of highly productive oil palms. Unfortunately, tissue culture sometimes accentuates the expression of detects in oil palm, particularly when embryogenesis is induced in particullar callus for prolonged periods. This research is conducted by taking individual tree sample of clone germplasm two years old. The purpose of this research is to molecular performance analysis of some oil palm clones based on SSR markers. A total of 30 trees oil palm clones were used for analysis. In this experiment, the DNA profile diversity was assessed using five loci of oil palm's specific SSR markers. The results of the experiment indicated out of 3 SSR markers (FR-0779, FR-3663 and FR-0782) showed monomorphic of PCR product and 2 SSR markers (FR-0783 and FR- 3745) showed polymorphic of PCR product. There are 10 total number of PCR product. These preliminary results demonstrated SSR marker can be used to evaluate genetic relatedness among trees of oil palm clones.