Molecular performance of commercial MTG variety oil palm based on RAPD markers

International Conference on Agriculture, Environment, and Food Security

农业科学;生态环境科学

Putri, L.A.P.^1 ; Setyo, I.E.^2 ; Basyuni, M.^3 ; Bayu, E.S.^1 ; Setiado, H.^1 ; Reynaldi, N.F.^1 ; Laia, H.^1 ; Puteri, S.A.K.^4 ; Arifiyanto, D.^5 ; Syahputra, I.^5

Department of Agrotechnology, Faculty of Agriculture, Universitas Sumatera Utara, Jln. Prof. A. Sofyan no.3, Medan, North Sumatera

20155, Indonesia^1

Pusat Penelitian Kelapa Sawit, Jl. B. Katamso 51 Kp., Baru Medan, Indonesia^2

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan

20155, Indonesia^3

PT. Rajawali Nusantara Indonesia (PT. RNI), Gedung RNI, Jl. Denpasar Raya, Kav D III, Kuningan, Jakarta

12950, Indonesia^4

PT. Socfin Indonesia, Jl. Kl Yos Sudarso No. 106, Medan, Sumatera Utara

20115, Indonesia^5

关键词: Bio-diesel fuel;    DNA profiles;    Genetic relatedness;    Genetic variants;    Germplasms;    Individual tree;    PCR products;    RAPD markers;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/122/1/012078/pdf
DOI  :  10.1088/1755-1315/122/1/012078

来源: IOP

【 摘 要 】

The oil palm, an economically important tree in Indonesia, has been one of the world's major sources of edible oil and a significant precursor of biodiesel fuel. This research is conducted by taking individual tree sample of commercial MTG variety germplasm oil palm one years old. The purpose of this research is to analyse molecular performance of some oil palm MTG variety based on RAPD markers. In this experiment, the DNA profile diversity was assessed using markers of oil palm's random RAPD markers (OPD-20, SB-19, OPM-01 and OPO-11). A total of 15 trees commercial MTG oil palm variety were used for analysis. The results of the experiment indicated out of 4 RAPD markers (OPD-20, SB-19, OPM-01 and OPO-11) showed polymorphic of PCR product. These preliminary results demonstrated RAPD marker can be used to evaluate genetic relatedness among trees of commercial MTG variety oil palm and detecting either genetic variants or mislabelled.