Differences in brain gene transcription profiles advocate for an important role of cognitive function in upstream migration and water obstacles crossing in European eel

BMC Genomics

Francoise Daverat²  Fabien Pierron³  Eric De Oliveira⁵  Luca Bargelloni¹  Gregory E Maes⁴  Jose Marti Pujolar⁶  Massimo Milan¹  Tomasz Podgorniak² 

[1] University of Padova, Viale dell’Università 16, Legnaro 35020, PD, Italy;Irstea Bordeaux, UR EABX, HYNES (Irstea – EDF R&D), 50 avenue de Verdun, Cestas 33612, Cedex, France;CNRS, EPOC, UMR 5805, Talence F-33400, France;Laboratory of Biodiversity and Evolutionary Genomics, University of Leuven (KU Leuven), Leuven B-3000, Belgium;EDF R&D LNHE, HYNES (Irstea-EDF R&D), 6, quai Watier, Bat Q, Chatou 78400, France;Department of Bioscience, Aarhus University, Ny Munkegade 114, Aarhus C DK-8000, Denmark

关键词: Fish brain;    Synaptic plasticity;    Microarray;    Water dams;    European eel;    Transcripomics;   

Others  :  1203967
DOI  :  10.1186/s12864-015-1589-y

 received in 2014-12-05, accepted in 2015-04-27,  发布年份 2015

【 摘 要 】

Background

European eel is a panmictic species, whose decline has been recorded since the last 20 years. Among human-induced environmental factors of decline, the impact of water dams during species migration is questioned. The main issue of this study was to pinpoint phenotypic traits that predisposed glass eels to successful passage by water barriers. The approach of the study was individual-centred and without any a priori hypothesis on traits involved in the putative obstacles selective pressure. We analyzed the transcription level of 14,913 genes.

Results

Transcriptome analysis of three tissues (brain, liver and muscle) from individuals sampled on three successive forebays separated by water obstacles indicated different gene transcription profiles in brain between the two upstream forebays. No differences in gene transcription levels were observed in liver and muscle samples among segments. A total of 26 genes were differentially transcribed in brain. These genes encode for, among others, keratins, cytokeratins, calcium binding proteins (S100 family), cofilin, calmodulin, claudin and thy-1 membrane glycoprotein. The functional analysis of these genes highlighted a putative role of cytoskeletal dynamics and synaptic plasticity in fish upstream migration.

Conclusion

Synaptic connections in brain are solicited while eels are climbing the obstacles with poorly designed fishways. Successful passage by such barriers can be related to spatial learning and spatial orientation abilities when fish is out of the water.

【 授权许可】

   
2015 Podgorniak et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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