Gene expression and genetic analysis reveal diverse causes of recessive self-compatibility in Brassica napus L.

BMC Genomics

Tingdong Fu²  Jinxiong Shen²  Jinxing Tu²  Guilong Zhou²  Changbin Gao²  Zhiquan Liu²  Chaozhi Ma²  Yong Yang²  Jianfeng Zhang¹  Wen Zhai² 

[1] Zhengzhou Tobacco Research Institute, Zhengzhou 450001, People’s Republic of China;National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China

关键词: Genetic analysis;    Gene expression;    S locus genes;    Self-incompatibility (SI);    Brassica napus;   

Others  :  1090453
DOI  :  10.1186/1471-2164-15-1037

 received in 2014-07-26, accepted in 2014-11-19,  发布年份 2014

【 摘 要 】

Background

Brassica napus (AACC) is self-compatible, although its ancestor species Brassica rapa (AA) and Brassica oleracea (CC) are self-incompatible. Most B.napus accessions have dominant self-compatibility (SC) resulting from an insertion of 3.6 kb in the promoter region of BnSCR-1 on the A genome, while recessive SC in B.napus has rarely been observed. Expression and cloning of SRK and SCR genes and genetic analysis were carried out to dissect bases of recessive SC in B.napus.

Results

Eleven accessions were screened to identify stable recessive SC and had the S genotype BnS-7 on the A genome and BnS-6 on the C genome similarly to BrS-29 and BoS-15, respectively. In eight SC accessions, BnSCR-7 and BnSCR-6 were nearly undetectable and harbored no structural mutations in the promoters, while SRK genes were expressed at normal levels and contained intact CDS, with the exception of BnSRK-7 in line C32. SRK and SCR genes were expressed normally but their CDSs had no mutations in three SC accessions. In self-incompatible S-1300 and 11 F₁ hybrids, SRK genes and BnSCR-1300 transcripts were present at high levels, while expression of the BnSCR-7 and BnSCR-6 were absent. Plants of S genotype S₁₃₀₀S₁₃₀₀ were completely SI, while SI phenotypes of S_BnS-7S_BnS-7 and S₁₃₀₀S_BnS-7 plants were segregated in BC₁ and F₂ populations.

Conclusions

The recessive SC in eight accessions is caused by the loss of function of BnSCR-7 and BnSCR-6 in pollen. Translational repression contributes to the recessive SC in three accessions, whose SRK and SCR genes were expressed normally and had identical CDSs to BrS-29 or BoS-15. SI in 11 F₁ hybrids relies on the expression of BnSCR-1300 rather than SRK genes. Other factor(s) independent of the S locus are involved in recessive SC. Therefore, diverse causes underlie recessive SC in B. napus, yielding insight into these complex mechanisms.

【 授权许可】

   
2014 Zhai et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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