Genetic similarity analysis of Vanda tricolor Lindley var. Suavis orchids using OPU 03 and OPU 16 RAPD marker

INTERNATIONAL BIOTECHNOLOGY CONFERENCE ON ESTATE CROPS 2017

Wibisono, Tony Haryo^1 ; Suratsih^1 ; Yulianti, E.^1 ; Sugiyarto, L.^1 ; Mercuriani, I.S.^1

Biology Education Department, Mathematics and Science Faculty, Yogyakarta State University, Jl. Karangmalang, Sleman, D.I., Yogyakarta

55281, Indonesia^1

关键词: Coconut water;    Electropherograms;    Genetic analysis;    Genetic similarities;    Random amplification of polymorphic;    RAPD markers;    Self pollination;    Software versions;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/183/1/012005/pdf
DOI  :  10.1088/1755-1315/183/1/012005

来源: IOP

【 摘 要 】

The purpose of this research is to understand the genetic similarity level of Vanda tricolor Lindley var. Suavis orchid plants that were regenerated from seeds of the same fruit/pod produced by self-pollination. The seeds were sown on New Phalaenopsis (NP) in vitro culture medium supplemented with 15 % coconut water (CW). Genetic similarity analysis was performed by PCR-based Random Amplification of Polymorphic DNA (PCR-RAPD) technique. The analysis was carried out at 16 months old regenerated plants after sowing (mas). Seven DNA samples were isolated using a modified Castillo method. It was then used to amplify (PCR) OPU 3 and OPU 16 primers. The electropherogram of PCR result was then converted to binary data and analysed using NTSYSpc software version 2.02. The genetic analysis result showed that the genetic similarity of 7 tested plants ranged between 0.4-0.8.