MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

BMC Microbiology

Roberto Meyer²  Robert Eduard Schaer²  Milton Galdino de Oliveira-Neto²  Paulo Cirino de Carvalho-Filho²  Heidiane Alves dos Santos²  Tatiane Santana Sales²  Gabriele Costa Rodrigues²  Lília Ferreira de Moura-Costa²  Soraya Castro Trindade²  Inara Barbosa de Oliveira Araújo²  Marcos da Costa Silva¹  Vera Lúcia Costa Vale¹  Andréia Pacheco de Souza² 

[1] Department of Exact and Earth Sciences (DCET), State University of Bahia (UNEB), Alagoinhas, Bahia, Brazil;Biointeraction Department - Immunology and Molecular Biology Laboratory ¿ Health Sciences Institute (ICS), Federal University of Bahia (UFBA), Av. Reitor Miguel Calmon, s/n; Vale do Canela, Salvador CEP 40040-040, Bahia, Brazil

关键词: MAPkinases;    Cytokines;    Corynebacterium pseudotuberculosis;   

Others  :  1140421
DOI  :  10.1186/s12866-014-0230-6

 received in 2014-04-04, accepted in 2014-08-19,  发布年份 2014

【 摘 要 】

Background

Caseous lymphadenitis (CL) is a contagious infectious disease of small ruminants caused by Corynebacterium pseudotuberculosis. Is characterized by the formation of abscesses in the lymph nodes and intestines of infected animals, induced by inflammatory cytokines. The production of cytokines, such as IL-10, TNF-?, IL-4 and IFN-?, is regulated by mitogen-activated protein kinase (MAPK) pathway activation. The present study investigated the involvement of MAPK pathways (MAPK p38, ERK 1 and ERK 2) with respect to the production of cytokines induced by antigens secreted by C. pseudotuberculosis over a 60-day course of infection. CBA mice (n?=?25) were divided into three groups and infected with 10² colony forming units (CFU) of attenuated strain T1, 10² CFU of virulent strain VD57 or sterile saline solution and euthanized after 30 or 60 days. Murine splenocytes were treated with specific inhibitors (MAPK p38 inhibitor, ERK 1/2 inhibitor or ERK 2 inhibitor) and cultured with secreted antigens obtained from pathogenic bacteria (SeT1 or SeVD57).

Results

The MAPK pathways evaluated were observed to be involved in the production of IL-10, under stimulation by secreted antigens, while the MAPK p38 and ERK 1 pathways were shown to be primarily involved in TNF-? production. By contrast, no involvement of the MAPK p38 and ERK 1 and 2 pathways was observed in IFN-? production, while the ERK 2 pathway demonstrated involvement in IL-4 production only in the mouse splenocytes infected with VD57 under stimulation by SeT1.

Conclusion

The authors hypothesize that MAPK p38 and ERK 1 pathways with respect to TNF-? production, as well as the MAPK p38 and ERK 1 and 2 pathways in relation to IL-10 production under infection by C. pseudotuberculosis are important regulators of cellular response. Additionally, the lack of the MAPK p38 and ERK 1/2 pathways in IFN-? production in infected CBA murine cells stimulated with the two secreted/excreted antigens, in IL-4 production showing involvement only via the ERK 2 pathway under stimulation by SeT1 antigen during 60-day infection period with the virulent strain, suggests that these pathways regulated the production of pro-inflammatory and regulatory cytokines in the splenic cells of CBA mice.

【 授权许可】

   
2014 de Souza et al.; licensee BioMed Central Ltd.

【 图 表 】

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