Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants

BMC Complementary and Alternative Medicine

Wanchai Assavalapsakul³  Jisnuson Svasti²  Preecha Puwaprisirisan¹  Tikamporn Yongvanich⁴  Pakorn Winayanuwattikun⁴  Chantragan Srisomsap²  Kriengsak Lirdprapamongkol²  Chutima Kaewpiboon⁴ 

[1] Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand;Laboratory of Biochemistry, Chulabhorn Research Institute, Vibhavadee Road, Bangkok, 10210, Thailand;Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand;Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand

关键词: Thai medicinal plants;    Lipase inhibitory;    Cytotoxic;    Antioxidant;    Antimicrobial;   

Others  :  1231503
DOI  :  10.1186/1472-6882-12-217

 received in 2012-07-20, accepted in 2012-11-04,  发布年份 2012

【 摘 要 】

Background

Traditional folk medicinal plants have recently become popular and are widely used for primary health care. Since Thailand has a great diversity of indigenous (medicinal) plant species, this research investigated 52 traditionally used species of Thai medicinal plants for their in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities.

Methods

The 55 dried samples, derived from the medicinally used parts of the 52 plant species were sequentially extracted by hexane, dichloromethane, ethanol and water. These 220 extracts were then screened for in vitro (i) cytotoxicity against four cell lines, derived from human lung (A549), breast (MDA-MB-231), cervical (KB3-1) and colon (SW480) cancers, using the MTT cytotoxicity assay; (ii) antioxidant activity, analyzed by measuring the scavenging activity of DPPH radicals; (iii) lipase inhibitory activity, determined from the hydrolytic reaction of p-nitrophenyllaurate with pancreatic lipase; and (iv) antimicrobial activity against three Gram-positive and two Gram-negative bacteria species plus one strain of yeast using the disc-diffusion method and determination of the minimum inhibitory concentration by the broth micro-dilution assay.

Results

The crude dichloromethane and/or ethanol extracts from four plant species showed an effective in vitro cytotoxic activity against the human cancer cell lines that was broadly similar to that of the specific chemotherapy drugs (etoposide, doxorubicin, vinblastine and oxaliplatin). In particular, this is the first report of the strong in vitro cytotoxic activity of Bauhinia strychnifolia vines. The tested tissue parts of only six plant species (Allium sativum, Cocoloba uvifera, Dolichandrone spathacea, Lumnitzera littorea, Sonneratia alba and Sonneratia caseolaris) showed promising potential antioxidant activity, whereas lipase inhibitory activity was only found in the ethanol extract from Coscinum fenestratum and this was weak at 17-fold lower than Orlistat, a known lipase inhibitor. The highest antimicrobial activity was observed in the extracts from S. alba and S. caseolaris against Pseudomonas aeruginosa and Candida albicans, respectively.

Conclusion

The Thai medicinal plant B. strychnifolia is first reported to exert strong in vitro cytotoxic activities against human cancer cell lines and warrants further enrichment and characterization. The broad spectrum of the biological activities from the studied plant extracts can be applied as the guideline for the selection of Thai medicinal plant species for further pharmacological and phytochemical investigations.

【 授权许可】

   
2012 Kaewpiboon et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Stevigny C, Bailly C, Quetin-Leclercq J: Cytotoxic and antitumor potentialities of aporphinoid alkaloids. Curr Med Chem Anticancer Agents 2005, 5:173-182.
• [2]Tang J, Feng Y, Tsao S, Wang N, Curtain R, Wang Y: Berberine and Coptidis Rhizoma as novel antineoplastic agents: A review of traditional use and biomedical investigations. J Ethnopharmacol 2009, 126:5-17.
• [3]Wungsintaweekul B, Umehara K, Miyase T, Noguchi H: Estrogenic and anti-estrogenic compounds from the Thai medicinal plant, Smilax corbularia (Smilacaceae). Phytochemistry 2011, 72:495-502.
• [4]Nadeem M, Rikhari HC, Kumar A, Palni LMS, Nandi SK: Taxol content in the bark of Himalayan Yew in relation to tree age and sex. Phytochemistry 2002, 60:627-631.
• [5]Anushia C, Sampathkumar P, Ramkumar L: Antibacterial and antioxidant activities in Cassia auriculata. Glob J Pharmacol 2009, 3:127-130.
• [6]Temkitthawon P, Viyoch J, Limpeanchob N, Pongamornkul W, Sirikul C, Kumpila A, Suwanborirux K, Ingkaninan K: Screening for phosphodiesterase inhibitory activity of Thai medicinal plants. J Ethanopharmacol 2008, 119:214-217.
• [7]Siriwatanametanon N, Fiebich BL, Efferth T, Prieto JM, Heinrich M: Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities. J Ethanopharmacol 2010, 130:196-207.
• [8]Farnsworth NR, Bunyapraphatsara N: Thai Medicinal Plant. Recommended for primary health care system Prachachon Company, Bangkok, Thailand; 1992.
• [9]Kaisoon O, Konczak I, Siriamornpun S: Potential health enhancing properties of edible flowers from Thailand. Food Res Int 2012, 46:563-571.
• [10]Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S: Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem 2007, 103:381-388.
• [11]Wongsa P, Chaiwarit J, Zamaludien A: In vitro screening of phenolic compounds, potential inhibition against α-amylase and α-glucosidase of culinary herbs in Thailand. Food Chem 2012, 131:964-971.
• [12]Sirinamarattana A, Bavovada R, Pengsuparp T, Pongthananikorn S: Pancreatic lipase inhibitory activity of Thai medicinal plants. J Pharmacol Res 2010, 3:2402-2403.
• [13]Alzoreky NS, Nakahara K: Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol 2003, 80:223-230.
• [14]Sakunpak A, Panichayupakaranant P: Antibacterial activity of Thai edible plants against gastrointestinal pathogenic bacteria and isolation of a new broad spectrum antibacterial polyisoprenylated benzophenone, chamuangone. Food Chem 2012, 130:826-831.
• [15]Bunyavejchewin S, Buasalee R: Mangrove Forest: ecology and mangrove plants. Department of national parks, wildlife and plant conservation, Bangkok; 2011.
• [16]Mothana RA, Lindequist U, Gruenert R, Bednarski PJ: Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra. BMC Complem altern M 2009, 9:7.
• [17]Kanintronkul Y, Worayuthakarn R, Thasana N, Winayanuwattikun P, Pattanapanyasat K, Surarit R, Ruchirawat S, Svasti J: Overcoming multidrug resistance in human lung cancer with novel benzo[α]quinolizin-4-ones. Anticancer Res 2011, 31:921-927.
• [18]Qusti SY, Abo-khatwa AN, Lahwa MAB: Screening of antioxidant activity and phenolic content of selected food items cited in the Holly Quran. J Biol Sci 2010, 2:40-51.
• [19]Kim HY, Kang MH: Screening of Korean medicinal plants for lipase inhibitory activity. Phytother Res 2005, 19:359-361.
• [20]Lachumy SJT, Zuraini Z, Sasidharan S: Antimicrobial activity and toxicity of methanol extract of Cassia fistula seeds. Res J Pharm Biol Chem Sci 2010, 1:391-398.
• [21]Vital PG, Rivera WL: Antimicrobial activity, cytotoxicity, and phytochemical screening of Voacanga globosa (Blanco) Merr. leaf extract (Apocynaceae). Asian Pac J Trop Med 2011, 4:824-828.
• [22]Londonkar R, Reddy VC, Kumar KA: Potential antibacterial and antifungal activity of Achyranthes aspera L. Rec Res Sci Tech 2011, 3:53-57.
• [23]Kuete V, Wiench B, Hegazy ME, Mohamed TA, Fankam AG, Shahat AA, Efferth T: Antibacterial activity and cytotoxicity of selected Egyptian medicinal plants. Planta Med 2012, 78:193-199.
• [24]Chamratpan S, Homchuen S: Ethnobotany in upper northeastern Thailand. Acta Hortic 2005, 675:67-74.
• [25]Bunluepuech K, Tewtrakul S: Anti-HIV-1 integrase activity of Thai medicinal plants in longevity preparations. Songklanakarin J Sci Technol 2011, 33:693-697.
• [26]Rojsanga P, Sukhthankar M, Krisanapun C, Gritsanapan W, Lawson DB, Baek SJ: In vitro anti-proliferative activity of alcoholic stem extract of Coscinium fenestratum in human colorectal cancer cells. Exp Thera Med 2010, 1:181-186.
• [27]Tee TT, Azimahtol HL: Induction of apoptosis by Eurycoma longifolia Jack. extracts. Anticancer Res 2005, 25:2205-2213.
• [28]Tee TT, Cheah YH, Hawariah LP: F16, a fraction from Eurycoma longifolia Jack extract, induces apoptosis via a caspase-9-independent manner in MCF-7 cells. Anticancer Res 2007, 27:3425-3430.
• [29]Kuo P-C, Damu AG, Lee K-H, Wu T-S: Cytotoxic and antimalarial constituents from the roots of Eurycoma longifolia. Bioorg Med Chem 2004, 12:537-544.
• [30]Supratman U, Fujita T, Akiyama K, Hayashi H, Murakami A, Sakai H, Koshimizu K, Ohigashi H: Anti-tumor promoting activity of bufadienolides from Kalanchoe pinnata and K. daigremontiana x tubiflora. Biosci Biotechnol Biochem 2001, 65:947-949.
• [31]Bukhari SB, Bhanger MI, Memon S: Antioxidative activity of extracts from Fenugreek seeds (Trigonella foenum-graecum). Pak J Anal Chem 2008, 9:78-83.
• [32]Bandaranayake WM: Traditional and medicinal uses of mangroves. Mangroves and Salt Marshes 1998, 2:133-148.
• [33]Agati G, Stefano G, Biricolti S, Tattini M: Mesophyll distribution of ‘antioxidant’ flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance. Ann Bot 2009, 104:853-861.
• [34]Close DC, McArthur C: Rethinking the role of many plant phenolics: protection from photodamage not herbivores? Oikos 2002, 99:166-172.
• [35]Ghasemzadeh A, Jaafar HZ, Rahmat A: Identification and concentration of some flavonoid components in Malaysian young ginger (Zingiber officinale Roscoe) varieties by a high performance liquid chromatography method. Molecules 2010, 15:6231-6243.
• [36]Kumar G, Jayaveera K, Kumar CA, Sanjay UP, Swamy BV, Kumar DK: Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop J Pharm Res 2007, 6:717-723.
• [37]Nair GM, Narasimhan S, Shiburaj S, Abraham TK: Antibacterial effects of Coscinium fenestratum. Fitoterapia 2005, 76:585-587.
• [38]Saad S, Taher M, Susanti D, Qaralleh H, Fadhlina Izyani Bt Awang A: In vitro antimicrobial activity of mangrove plant Sonneratia alba. Asian Pac J Trop Biomed 2012, 427-429.
• [39]Tao W, Xu M, Huang L, Miao S: Antibacterial activity of extracts from four mangrove species in vitro. Med Plant 2012, 3:38-41.
• [40]Mahadlek J, Phachamud T, Wessapun C: Antimicrobial studies of Sonneratia caseolaris using different agar diffusion method. Res J Pharm Biol Che Sci 2012, 3:404.
• [41]Arekemase MO, Oyeyiola GP, Aliyu MB: Antibacterial activity of Anacaridum occidentale on some enterotoxin producing bacteria. Int J Biol 2011, 3:92-99.
• [42]Vijayakumar AD, Kalaichelvan PT: Antioxidant and antimicrobial activity using different extracts of Anacardium occidentale L. Int J ChemTech Res 2011, 2:436-443.
• [43]Dahake AP, Joshi VD, Joshi AB: Antimicrobial screening of different extract of Anacardium occidentale Linn. leaves. Int J ChemTech Res 2009, 1:856-858.