Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs

BMC Genomics

Klaus Wimmers⁷  Pierre Mormède⁵  Aline Foury⁵  Ronald Klont⁴  Esra Kurt¹  Ludger Thölking⁶  Gary Evans²  Simon P Turner³  Richard B D’Eath³  Siriluck Ponsuksili⁷  Eduard Muráni⁷  Michael Oster⁷ 

[1] Optimeter, Oyaksitesi 1.kisim 11b blok da:4, Sefakoy, Istanbul, Turkey;PIC UK, 2 Kingston Business Park, Kingston Bagpuize, Oxfordshire OX13 5FE, UK;Animal & Veterinary Science Research Group, SRUC, West Mains Road, Edinburgh EH9 3JG, UK;Vion Food Group, Boseind 10, 5281 RM Boxtel Postbus 1, 5280 AA Boxtel, The Netherlands;Université Victor Segalen Bordeaux 2, PsyNuGen, UMR 1286 INRA, 33076 Bordeaux, France;PIC Germany, PIC Deutschland GmbH, Ratsteich 31, 24837 Schleswig, Germany;Leibniz Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany

关键词: Unfamiliar mixing;    Psychosocial stress;    Pigs;    Peripheral blood mononuclear cells (PBMC);    Microarray;    Immune system;   

Others  :  1098208
DOI  :  10.1186/1471-2164-15-967

 received in 2013-12-13, accepted in 2014-10-15,  发布年份 2014

【 摘 要 】

Background

Brain and immune system are linked in a bi-directional manner. To date, it remained largely unknown why immune components become suppressed, enhanced, or remain unaffected in relation to psychosocial stress. Therefore, we mixed unfamiliar pigs with different levels of aggressiveness. We separated castrated male and female pigs into psychosocially high- and low- stressed animals by skin lesions, plasma cortisol level, and creatine kinase activity obtained from agonistic behaviour associated with regrouping. Peripheral blood mononuclear cells (PBMC) were collected post-mortem and differential gene expression was assessed using the Affymetrix platform (n = 16).

Results

Relevant stress-dependent alterations were found only between female samples, but not between castrated male samples. Molecular routes related to TREM 1 signalling, dendritic cell maturation, IL-6 signalling, Toll-like receptor signalling, and IL-8 signalling were increased in high stressed females compared to low stressed females. This indicates a launch of immune effector molecules as a direct response. According to the shifts of transcripts encoding cell surface receptors (e.g. CD14, TLR2, TLR4, TREM1) the study highlights processes acting on pattern recognition, inflammation, and cell-cell communication.

Conclusions

The transcriptional response partly affected the degree of ‘stress responsiveness’, indicating that the high stressed females altered their signal transduction due to potential infections and injuries while fighting.

【 授权许可】

   
2014 Oster et al.; licensee BioMed Central Ltd.

【 图 表 】

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