Bovine serum albumin in saliva mediates grazing response in Leymus chinensis revealed by RNA sequencing

BMC Genomics

Gongshe Liu¹  Liqin Cheng¹  Shuangyan Chen¹  Lexin Zhang⁴  Xianjun Peng²  Xin Huang³ 

[1] Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, People's Republic of China;Graduate Schoo1 of the Chinese Academy of Sciences, Beijing, People's Republic of China;ShangHai Academy of Agricultural Sciences, Forest and Fruit Tree Research Institute, Shanghai, People's Republic of China;Heze Entry-Exit Inspection and Quarantine Bureau, Shandong, People's Republic of China

关键词: Cell oxidative status;    Apoptosis;    RNA-seq;    BSA deposition;    Grazing;    Leymus chinensis;   

Others  :  1127146
DOI  :  10.1186/1471-2164-15-1126

 received in 2014-03-25, accepted in 2014-12-03,  发布年份 2014

【 摘 要 】

Background

Sheepgrass (Leymus chinensis) is an important perennial forage grass across the Eurasian Steppe and is adaptable to various environmental conditions, but little is known about its molecular mechanism responding to grazing and BSA deposition. Because it has a large genome, RNA sequencing is expensive and impractical except for the next-generation sequencing (NGS) technology.

Results

In this study, NGS technology was employed to characterize de novo the transcriptome of sheepgrass after defoliation and grazing treatments and to identify differentially expressed genes (DEGs) responding to grazing and BSA deposition. We assembled more than 47 M high-quality reads into 120,426 contigs from seven sequenced libraries. Based on the assembled transcriptome, we detected 2,002 DEGs responding to BSA deposition during grazing. Enrichment analysis of Gene ontology (GO), EuKaryotic Orthologous Groups (KOG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed that the effects of grazing and BSA deposition involved more apoptosis and cell oxidative changes compared to defoliation. Analysis of DNA fragments, cell oxidative factors and the lengths of leaf scars after grazing provided physiological and morphological evidence that BSA deposition during grazing alters the oxidative and apoptotic status of cells.

Conclusions

This research greatly enriches sheepgrass transcriptome resources and grazing-stress-related genes, helping us to better understand the molecular mechanism of grazing in sheepgrass. The grazing-stress-related genes and pathways will be a valuable resource for further gene-phenotype studies.

【 授权许可】

   
2014 Huang et al.; licensee BioMed Central.

【 图 表 】

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