RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis

BMC Genomics

Paulino Martínez⁴  María Isabel Quiroga³  Ariadna Sitjà-Bobadilla⁶  María José Redondo⁶  Belén G Pardo⁴  Roberto Bermúdez⁵  Ana Paula Losada³  Peter W Harrison¹  Paolo Ronza³  Diego Robledo² 

[1] Department of Genetics, Evolution and Environment, University College London, London, UK;Departamento de Genética, Facultad de Biología (CIBUS), Universidad de Santiago de Compostela, Santiago de Compostela 15782, Spain;Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain;Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain;Departamento de Anatomía y Producción Animal, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain;Instituto de Acuicultura Torre de la Sal (IATS-CSIC), Ribera de Cabanes, Castellón 12595, Spain

关键词: Digestive function;    Cytoskeleton;    Erythropoiesis;    Apoptosis;    Immune response;    Enteromyxosis;    Enteromyxum scophthalmi;    Turbot;    Transcriptome;    RNA-seq;   

Others  :  1122644
DOI  :  10.1186/1471-2164-15-1149

 received in 2014-05-20, accepted in 2014-12-16,  发布年份 2014

【 摘 要 】

Background

Enteromyxosis caused by the intestinal myxozoan parasite Enteromyxum scophthalmi is a serious threat for turbot (Scophthalmus maximus, L.) aquaculture, causing severe catarrhal enteritis leading to a cachectic syndrome, with no therapeutic options available. There are still many aspects of host-parasite interaction and disease pathogenesis that are yet to be elucidated, and to date, no analysis of the transcriptomic changes induced by E. scophthalmi in turbot organs has been conducted. In this study, RNA-seq technology was applied to head kidney, spleen and pyloric caeca of severely infected turbot with the aim of furthering our understanding of the pathogenetic mechanisms and turbot immune response against enteromyxosis.

Results

A huge amount of information was generated with more than 23,000 identified genes in the three organs, amongst which 4,762 were differently expressed (DE) between infected and control fish. Associate gene functions were studied based on gene ontology terms and available literature, and the most interesting DE genes were classified into five categories: 1) immune and defence response; 2) apoptosis and cell proliferation; 3) iron metabolism and erythropoiesis; 4) cytoskeleton and extracellular matrix and 5) metabolism and digestive function. The analysis of down-regulated genes of the first category revealed evidences of a connexion failure between innate and adaptive immune response, especially represented by a high number of DE interferon-related genes in the three organs. Furthermore, we found an intense activation of local immune response at intestinal level that appeared exacerbated, whereas in kidney and spleen genes involved in adaptive immune response were mainly down-regulated. The apoptotic machinery was only clearly activated in pyloric caeca, while kidney and spleen showed a marked depression of genes related to erythropoiesis, probably related to disorders in iron homeostasis. The genetic signature of the causes and consequences of cachexia was also demonstrated by the down-regulation of the genes encoding structural proteins and those involved in the digestive metabolism.

Conclusions

This transcriptomic study has enabled us to gain a better understanding of the pathogenesis of enteromyxosis and identify a large number of DE target genes that bring us closer to the development of strategies designed to effectively combat this pathogen.

【 授权许可】

   
2014 Robledo et al.; licensee BioMed Central.

【 图 表 】

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