Acupuncture promotes white adipose tissue browning by inducing UCP1 expression on DIO mice

BMC Complementary and Alternative Medicine

Bing-Mei Zhu²  Bin Xu²  Jingxin Yue¹  Qian Li²  Suyun He²  Shuping Fu²  Hao Hong²  Sheng-Feng Lu²  Yang Wang²  Weixing Shen² 

[1] School of Information Technology, Nanjing University of Chinese Medicine, 210023 Nanjing, China;Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, 210023 Nanjing, China

关键词: White adipose tissue;    UCP1;    Obesity;    Browning;    Acupuncture;   

Others  :  1084652
DOI  :  10.1186/1472-6882-14-501

 received in 2014-01-23, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

To study the influence of acupuncture and its possible mechanism on white adipose tissue of high fat diet-induced obese.

Methods

Four-week-old C57BL/6 J mice were randomly divided into a normal diet group and a high-fat diet (HFD) group. After 8 weeks, the HFD mice were randomly divided into Electro-acupuncture (EA) group and control group. Mice in the EA group were electro-acupunctured, under physical restraint, on Zusanli (ST36) and Neiting (ST44) acupoints, while the mice in the control group were under physical restraint only. Body weight and food intake were monitored, and serum leptin, cholesterol and triglyceride levels were measured by using biochemistrical methods. The effect of EA on white adipose tissues (WAT) was assessed by qPCR, immunobloting, immunohistochemistry (IHC), immunoprecipitation and cold endurance experiment.

Results

The WAT/body weight ratio decreased (P < 0.05) in the EA group, albeit no significant difference on food consumption between EA and control groups. The difference in the darkness of Epi-WAT between EA and control groups could be distinguished visually. HE staining indicated that the EA mice had an increased number of UCP1-immunoreactive paucilocular adipocytes in their WAT. The expressions of brown adipose tissue (BAT) markers, including UCP1, COX4il and Nrtf1 were increased in the WAT of EA mice, acetylation of Pparγ was decreased by electro-acupuncture.

Conclusion

EA can remodel WAT to BAT through inducing UCP1 expression, and this may be one of the mechanisms by which acupuncture affects weight loss.

【 授权许可】

   
2014 Shen et al.; licensee BioMed Central.

【 图 表 】

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