Envelope protein gene based molecular characterization of Japanese encephalitis virus clinical isolates from West Bengal, India: a comparative approach with respect to SA14-14-2 live attenuated vaccine strain

BMC Infectious Diseases

Shyamalendu Chatterjee³  Subhra K Mukhopadhyay²  Bani K Pathak¹  Avishek Banik²  Arindam Sarkar³ 

[1] Department of Biotechnology, St. Xavier’s College, Kolkata, West Bengal, India;Department of Microbiology, The University of Burdwan, Golapbag, Burdwan, West Bengal, India;ICMR virus unit, GB- 4, 1st Floor, ID & BG Hospital, 57, Dr. S. C. Banerjee Road, Beliaghata, Kolkata 700010, West Bengal, India

关键词: West Bengal;    T-cell epitope;    Hydrophilicity;    Homology modeling;    Genotype I;    Genotype III;    Molecular phylogeny;    Envelope protein gene;    Japanese encephalitis virus;   

Others  :  1146339
DOI  :  10.1186/1471-2334-13-368

 received in 2013-03-22, accepted in 2013-07-30,  发布年份 2013

【 摘 要 】

Background

Increasing virulence of Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen is of grave concern because it causes a neurotrophic killer disease Japanese Encephalitis (JE) which, in turn, is responsible globally for viral acute encephalitis syndrome (AES). Despite the availability of vaccine, JE/AES cases and deaths have become regular features in the different rural districts of West Bengal (WB) state, India, indicating either the partial coverage of vaccine or the emergence of new strain of JEV. Therefore, a study was undertaken to characterize and compare the complete envelope (E) protein gene based molecular changes/patterns of JEVs circulating in WB.

Methods

Total of 98 AES case-patients’ samples were tested to detect the presence of JEV specific immunoglobulin M (IgM) antibody by Mac-ELISA method. Only JEV IgM negative samples with a history of ≤3 days’ illness were screened for virus isolation and RT-PCR. E gene sequences of JEV isolates were subjected to molecular phylogeny and immunoinformatics analysis.

Results

Present study confirmed JEV etiology in 39.7% and 29.1% of patients presenting ≤15 days’ febrile illness, as determined by Mac-ELISA and RT-PCR respectively. Phylogenetic analysis based on complete E gene sequences of JEV isolates showed the co-circulation of JEV genotype I (GI) with genotype III (GIII). This study also demonstrated that isolate-specific crucial amino acid substitutions were closely related to neurovirulence/neuroinvasiveness of JE. On the basis of immunoinformatics analysis, some substitutions were predicted to disrupt T-cell epitope immunogenicity/antigenicity that might largely influence the outcome of vaccine derived from JEV GIII SA14-14-2 strain and this has been observed in a previously vaccinated boy with mild JE/AES due to JEV GI infection.

Conclusions

Based on molecular evolutionary and bioinformatic approaches, we report evolution of JEV at a local level. Such naturally occurring evolution is likely to affect the disease profile and the vaccine efficacy to protect against JEV GI may demand careful evaluation.

【 授权许可】

   
2013 Sarkar et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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