Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli

BMC Gastroenterology

Aldo AM Lima³  Luciana R Bertolini²  Marcelo Bertolini²  Alexandre Havt¹  Paloma A Cavalcante¹  Mara MG Prata¹  Antônio VA Silva¹  Felipe AR Rodrigues¹  Hemerson YF Magalhães¹  Ila FN Lima¹  Josiane S Quetz¹  Elizabeth A Maga²  Eunice B Carvalho¹ 

[1] Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil;Department of Animal Science, University of California, Davis, CA, USA;School of Medicine, Health Science Center, University of Fortaleza, Fortaleza, Ceará, Brazil

关键词: Lysozyme;    Apoptosis;    Cells viability;    EAEC adherence;    Cells migration;    Cells proliferation;    Intestinal cells;   

Others  :  1121893
DOI  :  10.1186/1471-230X-12-106

 received in 2012-02-18, accepted in 2012-07-31,  发布年份 2012

【 摘 要 】

Background

Enteroaggregative Escherichia coli (EAEC) causes diarrhea, malnutrition and poor growth in children. Human breast milk decreases disease-causing bacteria by supplying nutrients and antimicrobial factors such as lysozyme. Goat milk with and without human lysozyme (HLZ) may improve the repair of intestinal barrier function damage induced by EAEC. This work investigates the effect of the milks on intestinal barrier function repair, bacterial adherence in Caco-2 and HEp-2 cells, intestinal cell proliferation, migration, viability and apoptosis in IEC-6 cells in the absence or presence of EAEC.

Methods

Rat intestinal epithelial cells (IEC-6, ATCC, Rockville, MD) were used for proliferation, migration and viability assays and human colon adenocarcinoma (Caco-2, ATCC, Rockville, MD) and human larynx carcinoma (HEp-2, ATCC, Rockville, MD) cells were used for bacterial adhesion assays. Goats expressing HLZ in their milk were generated and express HLZ in milk at concentration of 270 μg/ml . Cells were incubated with pasteurized milk from either transgenic goats expressing HLZ or non-transgenic control goats in the presence and absence of EAEC strain 042 (O44:H18).

Results

Cellular proliferation was significantly greater in the presence of both HLZ transgenic and control goat milk compared to cells with no milk. Cellular migration was significantly decreased in the presence of EAEC alone but was restored in the presence of milk. Milk from HLZ transgenic goats had significantly more migration compared to control milk. Both milks significantly reduced EAEC adhesion to Caco-2 cells and transgenic milk resulted in less colonization than control milk using a HEp-2 assay. Both milks had significantly increased cellular viability as well as less apoptosis in both the absence and presence of EAEC.

Conclusions

These data demonstrated that goat milk is able to repair intestinal barrier function damage induced by EAEC and that goat milk with a higher concentration of lysozyme offers additional protection.

【 授权许可】

   
2012 Carvalho et al.; licensee BioMed Central Ltd.

【 图 表 】

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