Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli

BMC Gastroenterology

Research Article

Marcelo Bertolini¹  Luciana R Bertolini¹  Elizabeth A Maga¹  Antônio VA Silva²  Ila FN Lima²  Mara MG Prata²  Hemerson YF Magalhães²  Alexandre Havt²  Eunice B Carvalho²  Josiane S Quetz²  Paloma A Cavalcante²  Felipe AR Rodrigues²  Aldo AM Lima³ 

[1] Department of Animal Science, University of California, Davis, CA, USA;Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil;Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil;School of Medicine, Health Science Center, University of Fortaleza, Fortaleza, Ceará, Brazil;

关键词: Intestinal cells;    Cells proliferation;    Cells migration;    EAEC adherence;    Cells viability;    Apoptosis;    Lysozyme;   

DOI  :  10.1186/1471-230X-12-106

 received in 2012-02-18, accepted in 2012-07-31,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEnteroaggregative Escherichia coli (EAEC) causes diarrhea, malnutrition and poor growth in children. Human breast milk decreases disease-causing bacteria by supplying nutrients and antimicrobial factors such as lysozyme. Goat milk with and without human lysozyme (HLZ) may improve the repair of intestinal barrier function damage induced by EAEC. This work investigates the effect of the milks on intestinal barrier function repair, bacterial adherence in Caco-2 and HEp-2 cells, intestinal cell proliferation, migration, viability and apoptosis in IEC-6 cells in the absence or presence of EAEC.MethodsRat intestinal epithelial cells (IEC-6, ATCC, Rockville, MD) were used for proliferation, migration and viability assays and human colon adenocarcinoma (Caco-2, ATCC, Rockville, MD) and human larynx carcinoma (HEp-2, ATCC, Rockville, MD) cells were used for bacterial adhesion assays. Goats expressing HLZ in their milk were generated and express HLZ in milk at concentration of 270 μg/ml . Cells were incubated with pasteurized milk from either transgenic goats expressing HLZ or non-transgenic control goats in the presence and absence of EAEC strain 042 (O44:H18).ResultsCellular proliferation was significantly greater in the presence of both HLZ transgenic and control goat milk compared to cells with no milk. Cellular migration was significantly decreased in the presence of EAEC alone but was restored in the presence of milk. Milk from HLZ transgenic goats had significantly more migration compared to control milk. Both milks significantly reduced EAEC adhesion to Caco-2 cells and transgenic milk resulted in less colonization than control milk using a HEp-2 assay. Both milks had significantly increased cellular viability as well as less apoptosis in both the absence and presence of EAEC.ConclusionsThese data demonstrated that goat milk is able to repair intestinal barrier function damage induced by EAEC and that goat milk with a higher concentration of lysozyme offers additional protection.

【 授权许可】

Unknown   
© Carvalho et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.