Evaluation of TUBEX-TF and OnSite Typhoid IgG/IgM Combo rapid tests to detect Salmonella enterica serovar Typhi infection during a typhoid outbreak in Harare, Zimbabwe

BMC Research Notes

Nyasha Chin’ombe²  Ngoni Xmas Majuru¹  Muchaneta Gudza-Mugabe¹  Vurayai Ruhanya²  Peter Gumbo¹  Sekesai Mtapuri-Zinyowera¹  Simba Tapera¹  Andrew Tarupiwa¹ 

[1] National Microbiology Reference Laboratory, Southerton, Harare, Zimbabwe;Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe, Avondale, Zimbabwe

关键词: Evaluation;    Rapid Tests;    Salmonella;    Typhoid fever;   

Others  :  1131353
DOI  :  10.1186/s13104-015-1015-1

 received in 2014-06-25, accepted in 2015-02-13,  发布年份 2015

【 摘 要 】

Background

Salmonella enterica serovar Typhi, the causative agent of typhoid, is endemic in most parts of the world especially in Africa. Reliable and rapid diagnosis of the bacterium is therefore critical for confirmation of all suspected typhoid cases. In many parts of Zimbabwe, laboratory capacity to isolate the microorganism by culture method as a way of diagnosis has limitations. In this study, two rapid serological kits, TUBEX-TF and OnSite Typhoid IgG/IgM Combo, were evaluated for possible expeditious diagnosis of Salmonella enterica serovar Typhi infection during a typhoid outbreak in Zimbabwe.

Methods

Blood was collected from patients with clinical signs and symptoms of typhoid in Harare, Zimbabwe during an outbreak. The standard culture method was used to diagnose the disease. Two rapid kits, the TUBEX-TF and OnSite Typhoid IgG/IgM Combo, were also used in parallel to diagnose typhoid according to manufacturers’ instructions. The diagnostic accuracy of the two kits was evaluated using the culture method as the gold standard.

Results

From all the cases diagnosed by the blood culture (n = 136), we enrolled 131 patients for the TUBEX-TF and 136 for the OnSite Typhoid IgG/IgM Combo tests. With the culture method as a reference standard, we found that TUBEX-TF test was 100% sensitive and 94.12% specific, with 63.16% positive and 100% negative predictive values (NPVs) and the OnSite Typhoid IgG/IgM Combo test was 100% sensitive and 94.35% specific, with 63.16% positive and 100% NPVs.

Conclusion

Our results indicated that TUBEX-TF and OnSite Typhoid IgG/IgM Combo rapid tests were useful tools for the rapid diagnosis of Salmonella enterica serovar Typhi infection during typhoid outbreaks in Zimbabwe. The tests performed very well in laboratory evaluations of blood culture-confirmed typhoid cases in Harare, Zimbabwe.

【 授权许可】

   
2015 Tarupiwa et al.; licensee BioMed Central.

【 参考文献 】

• [1]World Health Organization: Background Document: The Diagnosis, Treatment and Prevention of Typhoid Fever. Switzerland, Geneva; 2003.
• [2]Mogasale V, Desai SN, Mogasale VV, Park JK, Ochiai RL, Wierzba TF: Case fatality rate and length of hospital stay among patients with typhoid intestinal perforation in developing countries: a systematic literature review. PLoS One 2014, 9:e93784.
• [3]Butler T, Knight J, Nath SK, Speelman P, Roy SK, Azad MA: Typhoid fever complicated by intestinal perforation: a persisting fatal disease requiring surgical management. Rev Infect Dis 1985, 7:244-56.
• [4]Crump JA, Luby SP, Mintz ED: The global burden of typhoid fever. Bull World Health Organ 2004, 82:346-53.
• [5]Bhutta ZA, Threlfall J: Addressing the Global Disease Burden of Typhoid Fever. JAMA 2009, 302:898-9.
• [6]Baker S, Favorov M, Dougan G: Searching for the elusive typhoid diagnostic. BMC Infect Dis 2010, 10:45. BioMed Central Full Text
• [7]Lateef A, Olopoeniaa LA, Kingb AL: Widal agglutination test − 100 years later: still plagued by controversy. Postgrad Med J 2000, 76:80-4.
• [8]Olsen SJ, Pruckler J, Bibb W, Nguyen TM, Tran MT, Nguyen TM, et al.: Evaluation of rapid diagnostic tests for typhoid fever. J Clin Microbiol 2004, 42:1885-9.
• [9]Naheed A, Ram PK, Brooks WA, Mintz ED, Hossain MA, Parsons MM, et al.: Clinical value of Tubex and Typhidot rapid diagnostic tests for typhoid fever in an urban community clinic in Bangladesh. Diagn Microbiol Infect Dis 2008, 61:381-6.
• [10]Siba V, Horwood PF, Vanuga K, Wapling J, Sehuko R, Siba PM, et al.: Evaluation of Serological Diagnostic Tests using a Composite Reference Standard for Typhoid Fever in Papua New Guinea. Clin Vaccine Immunol 2012, 19:1833.
• [11]Kawano RL, Leano SA, Agdamag DM: Comparison of serological test kits for diagnosis of typhoid fever in the Philippines. J Clin Microbiol 2007, 45:246-7.
• [12]House D, Ho VA, Diep TS, Chinh NT, Bay PV, Vinh H, et al.: Antibodies to the Vi capsule of Salmonella Typhi in the serum of typhoid patients and healthy control subjects from a typhoid endemic region. J Infect Dev Ctries 2008, 2:308-12.
• [13]Lim PL, Tam FC, Cheong YM, Jegathesan M: One-step 2-minute test to detect typhoid-specific antibodies based on particle separation in tubes. J Clin Microbiol 1998, 1998(36):2271-8.
• [14]Keddy KH, Sooka A, Letsoalo ME, Hoyland G, Chaignat CL, Morrissey AB, et al.: Sensitivity and specificity of typhoid fever rapid antibody tests for laboratory diagnosis at two sub-Saharan African sites. Bull World Health Organ 2011, 89:640-7.
• [15]Fadeel MA, House BL, Wasfy MM, Klena JD, Habashy EE, Said MM, et al.: Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance. J Infect Dev Ctries 2011, 5:169-75.
• [16]CTK Biotech Inc. OnSite Typhoid IgG/IgM Combo Rapid Test-Cassette (Serum/Plasma/Whole blood). 2007. Manufacture’s manual. http://ctkbiotech.com/wp/wp-content/uploads/2014/07/PI-R0161C-Typhoid-IgG-IgM-Combo-Rev-E.pdf.