Protein Profile and Hemagglutination Activity of Pilli, an Adhesion Factor Causing Typhoid Fever by Salmonella typhi

International Conference on Food Science and Technology

Darmawati, S.^1 ; Ethica, S.N.^1 ; Dewi, S.S.^1

Department of Medical Laboratory Technology, Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang, Indonesia^1

关键词: Adhesion factors;    hemagglutination;    Human red blood cell;    Protein profiles;    Protein subunits;    Salmonella typhi;    SDS-PAGE analysis;    Typhoid fever;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/292/1/012049/pdf
DOI  :  10.1088/1755-1315/292/1/012049

来源: IOP

【 摘 要 】

The purposes of this study was to analyze protein profile of pilli member in two S. typhi isolates, BA07.4 and KD30.4, and assessing their hemagglutination activity on human red blood cells. Methods: Profile of pillin proteins extracted from two S. typhi isolates (BA07.4 and KD30.4) were observed using SDS-PAGE 12% method, hemagglutination activity of the isolated pilli proteins was tested on four erythrocyte of human blood groups ABO. Results showed that SDS-PAGE analysis on pillin proteins of S. typhi BA07.4 resulted two major protein subunits sized 87 and 42 kDa along with 14 minor ones. Menwhile, pillin proteins of S. typhi KD30.4 isolate showed two major protein subunits sized 87 and 42kDa along with 18 minor protein subunits observed on the SDS-PAGE. From the hemagglutination activity of S. typhi BA07.4 on the studied blood groups of A, B, AB, and 0 was 128, 2048, 64, and 2 HA (Hema-Agglutination), respectively. On the other hand, the hemagglutination activity of S. typhi KD30.4 on the studied blood groups of A, B, AB, and 0 was 8, 64, 1024, and 0 HA, respectively.