Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich Ascites tumor cells in vivo

BMC Complementary and Alternative Medicine

Meenakshi Sundaram Muthuraman¹  Satyajit Patra² 

[1] Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University Thanjavur, 613401, TamilNadu, India;Medical University of the Americas, Charlestown, Nevis, West Indies

关键词: Toxicology;    Apoptosis;    Antitumor;    Ehrlich ascites tumor;    Gracilaria edulis J. Ag;   

Others  :  1220499
DOI  :  10.1186/1472-6882-13-331

 received in 2013-04-03, accepted in 2013-11-22,  发布年份 2013

【 摘 要 】

Background

Marine environment is inestimable for their chemical and biological diversity and therefore is an extraordinary resource for the discovery of new anticancer drugs. Recent development in elucidation of the mechanism and therapeutic action of natural products helped to evaluate for their potential activity.

Methods

We evaluated Gracilaria edulis J. Ag (Brown algae), for its antitumor potential against the Ehrlich ascites tumor (EAT) in vivo and in vitro. Cytotoxicity evaluation of Ethanol Extract of Gracilaria edulis (EEGE) using EAT cells showed significant activity. In vitro studies indicated that EEGE cytotoxicity to EAT cells is mediated through its ability to produce reactive oxygen species (ROS) and therefore decreasing intracellular glutathione (GSH) levels may be attributed to oxidative stress.

Results

Apoptotic parameters including Annexin-V positive cells, increased levels of DNA fragmentation and increased caspase-2, caspase-3 and caspase-9 activities indicated the mechanism might be by inducing apoptosis. Intraperitoneally administration of EEGE to EAT-bearing mice helped to increase the lifespan of the animals significantly inhibited tumor growth and increased survival of mice. Extensive hematology, biochemistry and histopathological analysis of liver and kidney indicated that daily doses of EEGE up to 300 mg/kg for 35 days are well tolerated and did not cause hematotoxicity nor renal or hepatotoxicity.

Conclusion

Comprehensive antitumor analysis in animal model and in Ehrlich Ascites Tumor cells was done including biochemical, and pathological evaluations indicate antitumor activity of the extract and non toxic in vivo. It was evident that the mechanism explains the apoptotic activity of the algae extract.

【 授权许可】

   
2013 Patra and Muthuraman; licensee BioMed Central Ltd.

【 图 表 】

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