In ovo serial skeletal muscle diffusion tractography of the developing chick embryo using DTI: feasibility and correlation with histology

BMC Developmental Biology

E Mark Haacke¹  Jiani Hu¹  Zishu Zhang¹  Yongquan Ye¹  Yan Zhou²  Jia Hua²  Jianrong Xu²  Lianming Wu¹  Zachary DelProposto³  Zien Zhou² 

[1]Department of Radiology, Wayne State University, Detroit, MI, USA

[2]Department of Radiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

[3]Department of Radiology, Henry Ford Hospital, Detroit, MI, USA

关键词: Chick embryo;    Skeletal muscle;    Diffusion tensor imaging;    Magnetic resonance imaging;   

Others  :  1086155
DOI  :  10.1186/1471-213X-12-38

 received in 2012-06-09, accepted in 2012-12-14,  发布年份 2012

【 摘 要 】

Background

Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Diffusion tensor imaging (DTI), based on the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Yet embryonic motion degrades the in vivo acquisition of long-duration DTI. We used a dual-cooling technique to avoid motion artifact and aimed to investigate whether DTI can be used to monitor chick embryonic skeletal muscle development in ovo, and to investigate the correlation between quantitative DTI parameters fractional anisotropy (FA) and fiber length and quantitative histologic parameters fiber area percentage (FiberArea%) and limb length.

Results

From 84 normally developing chick embryos, 5 were randomly chosen each day from incubation days 5 to 18 and scanned using 3.0 Tesla magnetic resonance imaging. A dual-cooling technique is used before and during imaging. Eggs were cracked for making histological specimen after imaging. 3 eggs were serially imaged from days 5 to 18. We show that skeletal muscle fibers can be tracked in hind limb in DTI beginning with incubation day 8. Our data shows a good positive correlation between quantitative DTI and histologic parameters (FA vs FiberArea%: r= 0.943, p<0.0001; Fiber_length vs Limb_length: r=0.974, p<0.0001). The result of tracked fibers in DTI during incubation corresponds to the development of chick embryonic skeletal muscle as reported in the literature.

Conclusion

Diffusion tensor imaging can provide a noninvasive means of evaluating skeletal muscle development in ovo.

【 授权许可】

   
2012 Zhou et al.; licensee BioMed Central Ltd.

【 图 表 】

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