Multilocus variable-number tandem-repeat genotyping of Renibacterium salmoninarum, a bacterium causing bacterial kidney disease in salmonid fish

BMC Microbiology

Malcolm Hall⁴  Sarah J Weir⁴  I Stuart Wallace⁴  David Verner-Jeffreys³  Michael Snow⁵  Darryl McLennan⁴  Una McCarthy⁴  Edward J Feil¹  Duncan J Colquhoun²  Nicola Bain⁴  Iveta Matejusova⁴ 

[1] Department and Biology and Biochemistry, University of Bath, Bath BA1 7AY, UK;Norwegian Veterinary Institute, Pb 750, N-0106 Oslo, Norway;CEFAS, The Nothe, Barrack Road, Weymouth, Dorset DT4 8UB, UK;Marine Scotland Science, Victoria Road, P.O. Box 101, Aberdeen AB11 9DB, UK;WA Fisheries and Marine Research Laboratories, Department of Fisheries, Government of Western Australia, PO Box 20, North Beach, 6020 Perth, Australia

关键词: Salmonids;    Renibacterium;    Genotyping;    VNTR;    Bacterial kidney disease;   

Others  :  1142413
DOI  :  10.1186/1471-2180-13-285

 received in 2013-04-26, accepted in 2013-11-15,  发布年份 2013

【 摘 要 】

Background

Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum, is a bacterial disease of fish, which is both geographically widespread and difficult to control. Previously, application of various molecular typing methods has failed to reliably discriminate between R. salmoninarum isolates originating from different host species and geographic areas. The current study aimed to utilize multilocus variable number tandem repeats (VNTR) to investigate inter-strain variation of R. salmoninarum to establish whether host-specific populations exist in Atlantic salmon and rainbow trout respectively. Such information would be valuable in risk assessment of transmission of R. salmoninarum in a multispecies aquaculture environment.

Results

The present analysis utilizing sixteen VNTRs distinguished 17 different haplotypes amongst 41 R. salmoninarum isolates originating from Atlantic salmon and rainbow trout in Scotland, Norway and the US. The VNTR typing system revealed two well supported groups of R. salmoninarum haplotypes. The first group included R. salmoninarum isolates originating from both Atlantic salmon and rainbow trout circulating in Scottish and Norwegian aquaculture, in addition to the type strain ATCC33209^T originating from Chinook salmon in North America. The second group comprised isolates found exclusively in Atlantic salmon, of mainly wild origin, including isolates NCIB1114 and NCIB1116 associated with the original Dee disease in Scotland.

Conclusions

The present study confirmed that VNTR analysis can be successfully applied to discriminate R. salmoninarum strains. There was no clear distinction between isolates originating from Atlantic salmon and rainbow trout as several haplotypes in group 1 clustered together R. salmoninarum isolates from both species. These findings indicate a potential exchange of pathogens between Atlantic salmon and rainbow trout in Scottish and Norwegian aquaculture during the last 20 years. In a scenario of expansion of rainbow trout farming into the marine environment, appropriate biosecurity measures to minimize disease occurrence are advised. The present results also suggest that R. salmoninarum isolates circulating in European aquaculture over the last 20 years are genetically distant to the wild strains originally causing BKD in the rivers Dee and Spey.

【 授权许可】

   
2013 Matejusova et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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