Classification of ginseng berry (Panax ginseng C.A. MEYER) extract using 1H NMR spectroscopy and its inhibition of lipid accumulation in 3 T3-L1 cells

BMC Complementary and Alternative Medicine

Young Ock Kim²  Mariadhas Valan Arasu⁶  Hyung-Kyoon Choi⁴  Sae Won Na¹  Kee Hong Kim²  Young Chang Kim²  Hyung Don Kim²  Sang Won Lee²  Eun Suk Sohn⁵  Hae Ran Park²  Seung Ok Yang³ 

[1] College of Veterinary Medicine, ChonBuk National University, Jeonju-si, South Korea;Department of Medicinal Crop Research NIHHS RDA Eumsung, Chungbuk 369-873, Republic of Korea;Dapartment of food science and engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Republic of Korea;College of pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea;Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University, Chungju 380-701, South Korea;Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia

关键词: Maturation;    Metabolite profiling;    Obesity;    Panax ginseng berry;   

Others  :  1085330
DOI  :  10.1186/1472-6882-14-455

 received in 2014-08-25, accepted in 2014-11-13,  发布年份 2014

【 摘 要 】

Background

Panax ginseng is a famous traditional medicine in Korea for its beneficial effect on obesity, cardiac and liver associated diseases. The aim of this study was to investigate the metabolite in Panax ginseng (P. ginseng, Aralicaceae) berries depending on the ripen stages and evaluate its potential inhibition on adipocyte differentiation in 3 T3-L1 cells.

Methods

Different ripening stage samples of P. ginseng berry were analyzed through global metabolite profiling by NMR spectroscopy. Lipid accumulation in the cells was analyzed by Oil Red O staining.

Results

The PLS-DA clearly distinguished P. ginseng berry extract (PGBE) according to the partial ripe (PR), ripe(R) and fully ripe (FR) stage. Lipid accumulation of PGBE was examined by measuring triglyceride content and Oil-Red O staining. These results suggested that the FR stage of PGBE decrease in lipid accumulation during adipocyte differentiation and the amount of threonine, asparagine, fumarate, tyraine, tyrosine, and phenylalanine increased with longer ripening of ginseng berries.

Conclusion

Metabolite profiling of P. ginseng was identified by ¹H NMR spectra. P. ginseng extract efficiently inhibits adipogenesis in 3 T3-L1 adipocytes concluded that the P. ginseng has the antiobesity properties.

【 授权许可】

   
2014 Yang et al.; licensee BioMed Central Ltd.

【 图 表 】

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