Disintegration of wheat aleurone structure has an impact on the bioavailability of phenolic compounds and other phytochemicals as evidenced by altered urinary metabolite profile of diet-induced obese mice

Nutrition & Metabolism

Kati Hanhineva¹  Valérie Micard³  Kaisa Poutanen²  Hannu Mykkänen¹  Pekka Keski-Rahkonen¹  Otto-Ilari Savolainen¹  Natalia N Rosa²  Jenna Pekkinen¹ 

[1] Institute of Public Health and Clinical Nutrition, Clinical Nutrition, University of Eastern Finland, Kuopio Campus, P.O. Box 1627, Kuopio FI-70211, Finland;VTT Technical Research Centre of Finland, Espoo, Finland;JRU Agropolymers Engineering and Emerging Technologies (IATE 1208) SupAgro-INRA-UM2-CIRAD, 2 Place Pierre Viala, Montpellier cedex 1 34060, France

关键词: Metabolite profiling;    LC-MS;    Non-targeted metabolomics;    Microbial metabolism;    Ferulic acid;    Arabinoxylan;   

Others  :  802967
DOI  :  10.1186/1743-7075-11-1

 received in 2013-10-11, accepted in 2013-12-24,  发布年份 2014

【 摘 要 】

Background

Phenolic acids are covalently bound to the arabinoxylan fibre matrix of wheat aleurone layer. In order to be bioavailable they need to be released by endogenous or bacterial enzymes and absorbed within the intestinal lumen. The intestinal microbiota can metabolize phenolic acids and other food-born phytochemicals. However, the effect of structure of the cereal bran or aleurone layer on these processes is not comprehensively studied.

Methods

The structure of aleurone layer was modified either by dry-grinding or by enzymatic treatments with xylanase alone or in combination with feruloyl esterase. Diet induced obese C57BL6/J mice were fed with high-fat diets containing either pure ferulic acid, or one of the four differentially treated aleurone preparations for 8 weeks. The diets were designed to be isocaloric and to have similar macronutrient composition. The urinary metabolite profiles were investigated using non-targeted LC-qTOF-MS-metabolomics approach.

Results

The different dietary groups were clearly separated in the principal component analysis. Enzymatic processing of aleurone caused increased excretion of ferulic acid sulfate and glycine conjugates reflecting the increase in unbound form of readily soluble ferulic acid in the diet. The urinary metabolite profile of the diet groups containing native and cryo-ground aleurone was more intense with metabolites derived from microbial processing including hippuric acid, hydroxyl- and dihydroxyphenylpropionic acids. Furthermore, aleurone induced specific fingerprint on the urinary metabolite profile seen as excretion of benzoxazinoid metabolites, several small dicarboyxlic acids, and various small nitrogen containing compounds.

Conclusions

The structural modifications on wheat aleurone fraction resulted in altered metabolism of aleurone derived phenolic acids and other phytochemicals excreted in urine of diet-induced obese mice.

【 授权许可】

   
2014 Pekkinen et al.; licensee BioMed Central Ltd.

【 图 表 】

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