Changes of predominant species/biovars and sequence types of Brucella isolates, Inner Mongolia, China

BMC Infectious Diseases

Zeliang Chen⁴  Buyun Cui¹  Liuyu Huang³  Yaqin Yu²  Qing Zhen²  Zhoujia Wang³  Hai Jiang¹  Xiaoyan Zhou¹  Xitong Yuan³  Yufei Wang³  Yuehua Ke³  Yanfen Chen² 

[1] State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing, P.R. China;School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, P.R. China;Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing 100071, P.R. China;Research Center of Molecular Biology, Inner Mongolia Medical College, Hohhot, P.R. China

关键词: Multi locus sequence typing;    Biovar;    Genotype;    Brucella;   

Others  :  1145614
DOI  :  10.1186/1471-2334-13-514

 received in 2012-08-03, accepted in 2013-10-28,  发布年份 2013

【 摘 要 】

Background

Human brucellosis incidence in China was divided into 3 stages, high incidence (1950-1960s), decline (1970-1980s) and re-emergence (1990-2000s). Human brucellosis has been reported in all the 32 provinces, of which Inner Mongolia has the highest prevalence, accounting for over 40% of the cases in China. To investigate the etiology alteration of human brucellosis in Inner Mongolia, the species, biovars and genotypes of 60 Brucella isolates from this province were analyzed.

Methods

Species and biovars of the Brucella strains isolated from outbreaks were determined based on classical identification procedures. Strains were genotyped by multi locus sequence typing (MLST). Sequences of 9 housekeeping genes were obtained and sequence types were defined. The distribution of species, biovars and sequence types (STs) among the three incidence stages were analyzed and compared.

Results

The three stages of high incidence, decline and re-emergence were predominated by B. melitensis biovar 2 and 3, B. abortus biovar 3, and B. melitensis biovar 1, respectively, implying changes in the predominant biovars. Genotyping by MLST revealed a total of 14 STs. Nine STs (from ST28 to ST36), accounting for 64.3% of all the STs, were newly defined and different from those observed in other countries. Different STs were distributed among the three stages. ST8 was the most common ST in 1950-1960s and 1990-2000s, while ST2 was the most common in 1970-1980s.

Conclusions

The prevalence of biovars and sequence types of Brucella strains from Inner Mongolia has changed over time in the three stages. Compared with those from other countries, new sequence types of Brucella strains exist in China.

【 授权许可】

   
2013 Chen et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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