Genome-wide association study for birth weight in Nellore cattle points to previously described orthologous genes affecting human and bovine height

BMC Genetics

José F Garcia³  Tad S Sonstegard^1,10  Laercio R Porto Neto⁴  Marcos VGB da Silva⁵  Flávio S Schenkel⁶  Curtis P Van Tassell^1,10  John B Cole⁸  John C McEwan²  Johann Sölkner¹  Ana M Pérez O’Brien¹  Ludmilla B Zavarez⁹  Márcia C Matos⁹  Haroldo HR Neves⁹  Roberto Carvalheiro⁷  Adriana S do Carmo⁹  Yuri T Utsunomiya⁹ 

[1] Department of Sustainable Agricultural Systems, Division of Livestock Sciences, BOKU - University of Natural Resources and Life Sciences, Vienna, Austria;Centre for Reproduction and Genomics, AgResearch, Invermay, Mosgiel, New Zealand;Faculdade de Medicina Veterinária de Araçatuba, UNESP – Univ Estadual Paulista, Araçatuba, São Paulo, 16050-680, Brazil;School of Veterinary Sciences, The University of Queensland, Gatton, QLD 4343, Australia;Bioinformatics and Animal Genomics Laboratory, Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil;Centre for Genetic Improvement of Livestock, University of Guelph, Guelph, ON N1G2W1, Canada;GenSys Consultores Associados, Porto Alegre, 90680-000, Brazil;ARS-USDA - Agricultural Research Service - United States Department of Agriculture, Animal Improvement Programs Laboratory, Beltsville, Maryland, 20705, USA;Faculdade de Ciências Agrárias e Veterinárias, UNESP - Univ Estadual Paulista, Jaboticabal, São Paulo, 14884-900, Brazil;United States Department of Agriculture, Agricultural Research Service, Bovine Functional Genomics Laboratory, Beltsville, Maryland, 20705, USA

关键词: Stature;    Nellore cattle;    Bos primigenius indicus;    Birth weight;    GWAS;   

Others  :  1086964
DOI  :  10.1186/1471-2156-14-52

 received in 2012-12-19, accepted in 2013-06-05,  发布年份 2013

【 摘 要 】

Background

Birth weight (BW) is an economically important trait in beef cattle, and is associated with growth- and stature-related traits and calving difficulty. One region of the cattle genome, located on Bos primigenius taurus chromosome 14 (BTA14), has been previously shown to be associated with stature by multiple independent studies, and contains orthologous genes affecting human height. A genome-wide association study (GWAS) for BW in Brazilian Nellore cattle (Bos primigenius indicus) was performed using estimated breeding values (EBVs) of 654 progeny-tested bulls genotyped for over 777,000 single nucleotide polymorphisms (SNPs).

Results

The most significant SNP (rs133012258, P_GC = 1.34 × 10^-9), located at BTA14:25376827, explained 4.62% of the variance in BW EBVs. The surrounding 1 Mb region presented high identity with human, pig and mouse autosomes 8, 4 and 4, respectively, and contains the orthologous height genes PLAG1, CHCHD7, MOS, RPS20, LYN, RDHE2 (SDR16C5) and PENK. The region also overlapped 28 quantitative trait loci (QTLs) previously reported in literature by linkage mapping studies in cattle, including QTLs for birth weight, mature height, carcass weight, stature, pre-weaning average daily gain, calving ease, and gestation length.

Conclusions

This study presents the first GWAS applying a high-density SNP panel to identify putative chromosome regions affecting birth weight in Nellore cattle. These results suggest that the QTLs on BTA14 associated with body size in taurine cattle (Bos primigenius taurus) also affect birth weight and size in zebu cattle (Bos primigenius indicus).

【 授权许可】

   
2013 Utsunomiya et al.; licensee BioMed Central Ltd.

【 图 表 】

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