CardioNet: A human metabolic network suited for the study of cardiomyocyte metabolism

BMC Systems Biology

Hermann-Georg Holzhütter²  Vera Regitz-Zagrosek¹  Hugo Sanchez Ruderisch¹  Georgios Kararigas¹  Daniela Fliegner¹  Anja Karlstädt² 

[1]Center for Cardiovascular Research, Charité-Universitätsmedizin Berlin, 10115 Berlin, Hessische Straße 3-4, Germany

[2]Institute of Biochemistry, Charité-Universitätsmedizin Berlin, 10117 Berlin, Charitéplatz 1/ Virchowweg 6, Germany

关键词: Metabolism;    Efficency;    Cardiomyocyte;    Heart;    Flux balance;    Computational biology;   

Others  :  1143660
DOI  :  10.1186/1752-0509-6-114

 received in 2012-05-13, accepted in 2012-08-16,  发布年份 2012

【 摘 要 】

Background

Availability of oxygen and nutrients in the coronary circulation is a crucial determinant of cardiac performance. Nutrient composition of coronary blood may significantly vary in specific physiological and pathological conditions, for example, administration of special diets, long-term starvation, physical exercise or diabetes. Quantitative analysis of cardiac metabolism from a systems biology perspective may help to a better understanding of the relationship between nutrient supply and efficiency of metabolic processes required for an adequate cardiac output.

Results

Here we present CardioNet, the first large-scale reconstruction of the metabolic network of the human cardiomyocyte comprising 1793 metabolic reactions, including 560 transport processes in six compartments. We use flux-balance analysis to demonstrate the capability of the network to accomplish a set of 368 metabolic functions required for maintaining the structural and functional integrity of the cell. Taking the maintenance of ATP, biosynthesis of ceramide, cardiolipin and further important phospholipids as examples, we analyse how a changed supply of glucose, lactate, fatty acids and ketone bodies may influence the efficiency of these essential processes.

Conclusions

CardioNet is a functionally validated metabolic network of the human cardiomyocyte that enables theorectical studies of cellular metabolic processes crucial for the accomplishment of an adequate cardiac output.

【 授权许可】

   
2012 Karlstädt et al; licensee BioMed Central Ltd.

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