Novel antiviral activity of baicalein against dengue virus

BMC Complementary and Alternative Medicine

Sazaly AbuBakar²  Mohd Rais Mustafa¹  Pooi-Fong Wong¹  Sing-Sin Sam²  Boon-Teong Teoh²  Keivan Zandi² 

[1] Department of Pharmacology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia;Department of Medical Microbiology, Tropical Infectious Disease Research and Education Center (TIDREC), Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia

关键词: Baicalein;    Flavonoid;    Dengue;    Antiviral;    Flavivirus;    Infectious disease;   

Others  :  1231506
DOI  :  10.1186/1472-6882-12-214

 received in 2012-08-24, accepted in 2012-11-04,  发布年份 2012

【 摘 要 】

Background

Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits.

Methods

In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR.

Results

Baicalein inhibited DENV-2 replication in Vero cells with IC₅₀= 6.46 μg/mL and SI= 17.8 when added after adsorption to the cells. The IC₅₀ against DENV-2 was 5.39 μg/mL and SI= 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC ₅₀= 1.55 μg/mL and showed anti-adsorption effect with IC₅₀ = 7.14 μg/mL.

Conclusions

Findings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV.

【 授权许可】

   
2012 Zandi et al.; licensee BioMed Central Ltd.

【 图 表 】

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