Novel antiviral activity of baicalein against dengue virus

BMC Complementary and Alternative Medicine

Research Article

Boon-Teong Teoh¹  Sing-Sin Sam¹  Keivan Zandi¹  Sazaly AbuBakar¹  Pooi-Fong Wong²  Mohd Rais Mustafa² 

[1] Department of Medical Microbiology, Tropical Infectious Disease Research and Education Center (TIDREC), Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia;Department of Pharmacology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia;

关键词: Infectious disease;    Flavivirus;    Antiviral;    Dengue;    Flavonoid;    Baicalein;   

DOI  :  10.1186/1472-6882-12-214

 received in 2012-08-24, accepted in 2012-11-04,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits.MethodsIn the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR.ResultsBaicalein inhibited DENV-2 replication in Vero cells with IC50= 6.46 μg/mL and SI= 17.8 when added after adsorption to the cells. The IC50 against DENV-2 was 5.39 μg/mL and SI= 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC 50= 1.55 μg/mL and showed anti-adsorption effect with IC50 = 7.14 μg/mL.ConclusionsFindings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV.

【 授权许可】

Unknown   
© Zandi et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.