The ability of Abelmoschus manihot L. leaf extract in scavenging of free radical DPPH and total flavonoid determination

International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor in Quality Control of Pharmaceutical Products

Sudewi, S.^1 ; Lolo, W.A.^1 ; Warongan, M.^1 ; Rifai, Y.^2 ; Rante, H.^2

Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Manado, Indonesia^1

Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia^2

关键词: Abelmoschus manihot;    Active compounds;    Anti-oxidant activities;    Flavonoid;    Free radical scavenging;    Free-radical activity;    Leaf extracts;    Total flavonoids;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/259/1/012020/pdf
DOI  :  10.1088/1757-899X/259/1/012020

来源: IOP

【 摘 要 】

Abelmoschus manihot L. has reported to have flavonoids content. This study aims were to determine the ability of A. manihot extract in counteracting free radical DPPH and determine the content of total flavonoids. A. manihot leaf was taken from 2 regions in North Sulawesi, namely Tomohon and Kotamobagu. The maceration was carried out to extract the active compound in a 96% ethanol solvent. Free radical scavenging analysis was carried out by DPPH and determination of its total flavonoid in the extract was measured using spectrophotometri method. The results showed that A. manihot extract from Tomohon and Kotamobagu could counteract free radical of DPPH with value of free radical activity of 88.151 and 88.801 %, respectively. A. manihot leaf from Kotamobagu has higher total flavonoids content 61.763 mg/g compare to Tomohon 46.679 mg/g which presented as quercetin. A. manihot has antioxidant activity.