Neuropeptides encoded by the genomes of the Akoya pearl oyster Pinctata fucata and Pacific oyster Crassostrea gigas: a bioinformatic and peptidomic survey

BMC Genomics

Scott F Cummins¹  Joel Henry³  Abigail Elizur¹  Wayne A O’Connor⁴  Manzar Sohail¹  Min Zhao¹  Tianfang Wang¹  Bronwyn A Rotgans¹  Pascal Favrel²  Michael J Stewart¹ 

[1] School of Science and Education, Genecology Research Center, University of the Sunshine Coast, Maroochydore DC, Queensland 4558, Australia;CNRS UMR 7208, BOREA, Caen, France;Université de Caen Basse-Normandie, Biologie des ORganismes et Ecosystèmes Aquatiques (BOREA), Caen 14032, France;Port Stephens Fisheries Institute, Locked Bag 1, Nelson Bay, New South Wales 2315, Australia

关键词: Neuropeptides;    Mass spectrometry;    High-performance liquid chromatography;    Gonadotropin-releasing hormone;    Feed circuit activating peptide;    Egg-laying hormone;    Circular dichroism;    Molluscs;    Crassostrea gigas;    Pinctada fucata;   

Others  :  1139337
DOI  :  10.1186/1471-2164-15-840

 received in 2014-05-19, accepted in 2014-09-03,  发布年份 2014

【 摘 要 】

Background

Oysters impart significant socio-ecological benefits from primary production of food supply, to estuarine ecosystems via reduction of water column nutrients, plankton and seston biomass. Little though is known at the molecular level of what genes are responsible for how oysters reproduce, filter nutrients, survive stressful physiological events and form reef communities. Neuropeptides represent a diverse class of chemical messengers, instrumental in orchestrating these complex physiological events in other species.

Results

By a combination of in silico data mining and peptide analysis of ganglia, 74 putative neuropeptide genes were identified from genome and transcriptome databases of the Akoya pearl oyster, Pinctata fucata and the Pacific oyster, Crassostrea gigas, encoding precursors for over 300 predicted bioactive peptide products, including three newly identified neuropeptide precursors PFGx8amide, RxIamide and Wx3Yamide. Our findings also include a gene for the gonadotropin-releasing hormone (GnRH) and two egg-laying hormones (ELH) which were identified from both oysters. Multiple sequence alignments and phylogenetic analysis supports similar global organization of these mature peptides. Computer-based peptide modeling of the molecular tertiary structures of ELH highlights the structural homologies within ELH family, which may facilitate ELH activity leading to the release of gametes.

Conclusion

Our analysis demonstrates that oysters possess conserved molluscan neuropeptide domains and overall precursor organization whilst highlighting many previously unrecognized bivalve idiosyncrasies. This genomic analysis provides a solid foundation from which further studies aimed at the functional characterization of these molluscan neuropeptides can be conducted to further stimulate advances in understanding the ecology and cultivation of oysters.

【 授权许可】

   
2014 Stewart et al.; licensee BioMed Central Ltd.

【 图 表 】

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